TRANSFORMATION OF MOUSE BONE-MARROW CELLS BY TRANSFECTION WITH A HUMAN ONCOGENE RELATED TO C-MYC IS ASSOCIATED WITH THE ENDOGENOUS PRODUCTION OF MACROPHAGE COLONY STIMULATING FACTOR-I
TRANSFORMATION OF MOUSE BONE-MARROW CELLS BY TRANSFECTION WITH A HUMAN ONCOGENE RELATED TO C-MYC IS ASSOCIATED WITH THE ENDOGENOUS PRODUCTION OF MACROPHAGE COLONY STIMULATING FACTOR-I
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DOI：
10.1002/jcp.1041250307
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发表时间：
1985-01-01
影响因子：
5.6
通讯作者：
WALKER, WS
中科院分区：
文献类型：
--
作者：
SKLAR, MD;TEREBA, A;WALKER, WS
我们最近通过将新发现的人类基因R-myc与c-myc癌基因同源的小鼠骨髓细胞导入巨噬细胞前体细胞，获得了一系列转化的细胞系。在这份报告中，我们表明这些细胞系具有单核吞噬细胞谱系的一些特征。具体地说，所有细胞系均具有巨噬细胞或单核细胞样形态，含有非特异性酯酶，对乳胶珠具有吞噬作用，分泌溶菌酶，携带Mac-1抗原，并含有少数具有FC受体的细胞。然而，只有一个单核细胞克隆具有相当数量的补体受体1的细胞，并且没有一个细胞对抗体或补体包被颗粒具有吞噬功能，也没有一个细胞能够结构性地分泌白细胞介素1。所有这些细胞株都能分泌一种生长因子，支持骨髓巨噬细胞的体外增殖。放射免疫分析和受体结合研究表明，该因子为集落刺激因子1。
We recently derived a series of transformed cell lines by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with a newly described human gene, R-myc, which has homology to the c-myc oncogene. In this report, we show that these lines share some features characteristic of cells of the mononuclear phagocyte lineage. Specifically, all cell lines had macrophage- or monocytelike morphology, contained nonspecific esterase, were phagocytic for latex beads, secreted lysozyme, bore the Mac-1 antigen, and contained a minority of cells with Fc recpetors. However, only a single monocytelike clone had appreciable numbers of cells which bore complement receptor 1, and none were phagocytic for antibody or complement-coated particles, or constitutively secreted Interleukin-1. All these cell lines secreted a growth factor capable of supporting the in vitro proliferation of bone marrow macrophages. Radioimmunoassay and receptor binding studies indicate that this factor is colony stimulating factor 1.