Action of rat prolactin on plasma somatomedin levels in the rat and on somatomedin release from perfused rat liver.
Action of rat prolactin on plasma somatomedin levels in the rat and on somatomedin release from perfused rat liver.
复制标题
大鼠催乳素对大鼠血浆生长调节素水平和灌注大鼠肝脏生长调节素释放的作用。
DOI：
10.1677/joe.0.0750137
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
R. Milner
中科院分区：
文献类型：
--
作者：
D. Hill;M. Francis;R. Milner
大鼠催乳素在50 ng/ml灌注介质的浓度刺激生产的生长调节素样活性（SLA）从灌注正常大鼠的肝脏。该效应在11.00 h进行的灌注中得到证实，其中大鼠催乳素引起平均（+/- S.E.M.）与对照组相比，体外大鼠肋软骨对[35 S]硫酸盐的摄取增加了64 +/- 14%，但在15.00 h时未观察到影响。在11.00 h时，未检测到大鼠催乳素对切除垂体的大鼠肝脏的影响。垂体切除和假手术的大鼠给予5次静脉注射50微克大鼠催乳素或类似体积的激素溶剂，间隔12小时。血浆生长调节素活性（SMA）和软骨代谢，测定的放射性硫酸盐和胸苷肋软骨在体外的摄取，在垂体切除大鼠催乳素或激素溶剂的动物相似。给予大鼠催乳素的假手术大鼠与对照动物相比，血浆SMA和软骨代谢显着增加。大鼠肝脏响应大鼠催乳素而产生SLA可能与特定肝脏催乳受体的密度有关，因为这些受体在垂体切除动物中不存在或仅少量存在。
Rat prolactin at a concentration of 50 ng/ml perfusion medium stimulated the production of somatomedin-like activity (SLA) from the perfused liver of normal rats. The effect was demonstrable in perfusions performed at 11.00 h in which rat prolactin caused a mean (+/- S.E.M.) increase in the uptake of [35S]sulphate into rat costal cartilage in vitro of 64 +/- 14% in comparison with controls, but at 15.00 h no effect was observed. No effect or rat prolactin on hypophysectomized rat liver was detectable at 11.00 h. Hypophysectomized and sham-operated rats were given five intravenous injections of 50 microgram rat prolactin or a similar volume of hormone solvent at 12 h intervals. Plasma somatomedin activity (SMA) and cartilage metabolism, measured by the uptake of radioactive sulphate and thymidine by costal cartilage in vitro, were similar in hypophysectomized animals given rat prolactin or hormone solvent. Sham-operated rats given rat prolactin showed a significant increase of plasma SMA and cartilage metabolism compared with control animals. The production of SLA by rat liver in response to rat prolactin may be related to the density of specific hepatic lactogenic receptors, since these are absent or present only in low numbers in hypophysectomized animals.