Green chemistry fabrication of durable antimicrobial peptide-immobilized silk fibroin films for accelerated full-thickness wound healing
Green chemistry fabrication of durable antimicrobial peptide-immobilized silk fibroin films for accelerated full-thickness wound healing
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DOI：
10.1016/j.mtchem.2023.101468
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发表时间：
2023-04
影响因子：
7.3
通讯作者：
R. Si;W. Chen;J. Chen;Y. Yang;W. Zhou;Q. Zhang;C. Chen;B. Han
中科院分区：
文献类型：
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作者：
R. Si;W. Chen;J. Chen;Y. Yang;W. Zhou;Q. Zhang;C. Chen;B. Han
丝素蛋白(SF)因其优异的生物性能和力学性能，在创面敷料方面得到了广泛的研究。然而，形成具有持久和优异的抗菌性能以及生物相容性的SF膜仍然是一个挑战。本文将海洋来源的抗菌肽放线菌素X2(Ac.X2)固定在SF纤维和Ac上。采用各种绿色化学方法进一步制备了X2固定的SF(ASF)薄膜。固定化Ac的量。X2可以通过其在443 nm处的特征紫外-可见吸收峰来精确定量。傅里叶变换红外光谱和固体~(13)C核磁共振谱都表明，AC的α/β环上存在内酯键。X2与SF的氨基共价接枝，大大提高了固定化程度。ASF膜对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌(MRSA)表现出良好的抗菌活性，没有生物被膜形成。此外，ASF膜具有合适的降解率，良好的血液相容性，并在体外生物相容性试验中降低了细胞毒性。在体内大鼠全层创面愈合实验中，与对照组和SF创面敷料相比，ASF膜能有效地促进皮肤再生，促进创面血管生成，恢复皮肤功能。这些结果表明，多功能ASF膜可能是一种潜在的临床应用创面敷料。
Silk fibroin (SF) has been intensively explored for wound dressings due to its excellent biological and mechanical properties. However, forming SF films with a combination of durable and excellent antibacterial properties as well as biocompatibility is still a challenge. Herein, a marine-derived antimicrobial peptide, actinomycin X2 (Ac.X2), was immobilized onto SF fibers and Ac. X2-immobilized SF (ASF) films was further prepared using all green chemical approaches. The immobilized amount of Ac. X2 can be precisely quantified by its characteristic UV–visible absorption peak at 443 nm. Both Fourier transform infrared spectroscopy (FTIR) and solid-state13C NMR spectra revealed that the lactone bond on the α/β rings of Ac. X2 was covalently grafted with the amino groups of SF, which greatly improved the degree of immobilization. The ASF film exhibited excellent antimicrobial activity againstStaphylococcus aureusand Methicillin-resistantS.aureus(MRSA), with no biofilm formation. Furthermore, the ASF film showed a suitable degradation rate, good hemocompatibility, and reduced cytotoxicity in vitro biocompatibility assay. In vivo rat full-thickness wound healing experiment, the ASF film exhibited effectively promote skin regeneration, wound vascularization, and restore skin function compared with the control and SF wound dressings. These results suggest that the versatile ASF film would be a potential wound dressing for clinical application.