Abundance of metalloprotease FtsH12 modulates chloroplast development in Arabidopsis thaliana.
Abundance of metalloprotease FtsH12 modulates chloroplast development in Arabidopsis thaliana.
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DOI：
10.1093/jxb/eraa550
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发表时间：
2021-04-13
影响因子：
6.9
通讯作者：
Funk C
中科院分区：
文献类型：
--
作者：
Mielke K;Wagner R;Mishra LS;Demir F;Perrar A;Huesgen PF;Funk C
ATP依赖的金属蛋白酶FtsH 12（复性温度敏感蛋白H12）被认为参与异聚马达复合物，驱动蛋白质易位到叶绿体中。FtsH 12在前质体、幼苗、叶片和根中进行免疫检测。Myc标记的FtsH 12在其天然启动子下的表达允许鉴定FtsH 11，2，4和5，以及质体NAD-苹果酸脱氢酶，这是建议的进口电机复合物中的六个相互作用伙伴中的五个。FTSH 12丰度降低的拟南芥突变体幼苗表现出苍白的子叶和小的，变形的叶绿体与改变的类囊体结构。成熟的植株保留了这些叶绿体缺陷，导致略微杂色的叶片和较低的叶绿素含量。无标记蛋白质组学显示FTSH 12敲除幼苗的蛋白质组组成发生了强烈变化，反映了质体发育受损。内叶绿体膜（TIC）蛋白输入复合物上的易位子的组成被改变，与协调减少的FtsH 12-FtsHi复合物亚基和积累的1 MDa TIC复合物亚基TIC 56，TIC 214和TIC 22-III。FTSH 12过表达株没有表现出明显的表型，但在蛋白质组上仍表现出明显的差异。N-末端分析进一步证明了无论FTSH 12丰度如何，质体输入蛋白质的正常蛋白水解成熟。总之，我们的数据表明，FtsH 12在幼苗发育过程中具有最高的影响，它的丰度改变了质体进口机械和损害叶绿体发育。拟南芥中金属蛋白酶FtsH 12丰度的调节导致质体进口机器的组成改变，这反过来又影响功能叶绿体的发育。
The ATP-dependent metalloprotease FtsH12 (filamentation temperature sensitive protein H 12) has been suggested to participate in a heteromeric motor complex, driving protein translocation into the chloroplast. FtsH12 was immuno-detected in proplastids, seedlings, leaves, and roots. Expression of Myc-tagged FtsH12 under its native promotor allowed identification of FtsHi1, 2, 4, and 5, and plastidic NAD-malate dehydrogenase, five of the six interaction partners in the suggested import motor complex. Arabidopsis thaliana mutant seedlings with reduced FTSH12 abundance exhibited pale cotyledons and small, deformed chloroplasts with altered thylakoid structure. Mature plants retained these chloroplast defects, resulting in slightly variegated leaves and lower chlorophyll content. Label-free proteomics revealed strong changes in the proteome composition of FTSH12 knock-down seedlings, reflecting impaired plastid development. The composition of the translocon on the inner chloroplast membrane (TIC) protein import complex was altered, with coordinated reduction of the FtsH12-FtsHi complex subunits and accumulation of the 1 MDa TIC complex subunits TIC56, TIC214 and TIC22-III. FTSH12 overexpressor lines showed no obvious phenotype, but still displayed distinct differences in their proteome. N-terminome analyses further demonstrated normal proteolytic maturation of plastid-imported proteins irrespective of FTSH12 abundance. Together, our data suggest that FtsH12 has highest impact during seedling development; its abundance alters the plastid import machinery and impairs chloroplast development. Modulation of metalloprotease FtsH12 abundance in Arabidopsis results in altered composition of the plastid import machinery, which in turn affects the development of functional chloroplasts.
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影响因子：
14.9
作者：
Deutsch EW;Csordas A;Sun Z;Jarnuczak A;Perez-Riverol Y;Ternent T;Campbell DS;Bernal-Llinares M;Okuda S;Kawano S;Moritz RL;Carver JJ;Wang M;Ishihama Y;Bandeira N;Hermjakob H;Vizcaíno JA
通讯作者：
Vizcaíno JA
影响因子：
6.9
作者：
Fryer, MJ;Oxborough, K;Baker, NR
通讯作者：
Baker, NR
影响因子：
10.5
作者：
Kubo, M;Udagawa, M;Demura, T
通讯作者：
Demura, T
影响因子：
11.6
作者：
Lee, Sookjin;Lee, Dong Wook;Hwang, Inhwan
通讯作者：
Hwang, Inhwan
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y