Developing insights into the mechanisms of evolution of bacterial pathogens from whole-genome sequences.
Developing insights into the mechanisms of evolution of bacterial pathogens from whole-genome sequences.
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DOI：
10.2217/fmb.12.108
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发表时间：
2012-11
影响因子：
3.1
通讯作者：
Bentley SD
中科院分区：
文献类型：
--
作者：
Bryant J;Chewapreecha C;Bentley SD
细菌病原体种群的进化可以通过多种方式检测，包括表型测试，例如代谢活性、抗血清反应和耐药性，以及测量染色体结构、重复基因座和个体基因序列变异的基因型测试。虽然信息丰富，但这些方法仅捕获总变化的一小部分，因此分辨率有限。测序技术的进步使得捕获每个研究样本的全基因组序列变异成为可能，从而提供了检测基因组中所有位置的所有变化（从单核苷酸变化到大规模插入和缺失）的潜力。在这篇综述中，我们重点关注应用了这种强大的新方法的最新工作，并总结了这使我们了解细菌病原体如何进化的细节所取得的一些进展。
Evolution of bacterial pathogen populations has been detected in a variety of ways including phenotypic tests, such as metabolic activity, reaction to antisera and drug resistance and genotypic tests that measure variation in chromosome structure, repetitive loci and individual gene sequences. While informative, these methods only capture a small subset of the total variation and, therefore, have limited resolution. Advances in sequencing technologies have made it feasible to capture whole-genome sequence variation for each sample under study, providing the potential to detect all changes at all positions in the genome from single nucleotide changes to large-scale insertions and deletions. In this review, we focus on recent work that has applied this powerful new approach and summarize some of the advances that this has brought in our understanding of the details of how bacterial pathogens evolve.