Isolation and structure elucidation of procyanidin oligomers from saskatoon berries (Amelanchier alnifolia)
Isolation and structure elucidation of procyanidin oligomers from saskatoon berries (Amelanchier alnifolia)
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DOI：
10.1021/jf062441t
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发表时间：
2007-01-10
影响因子：
6.1
通讯作者：
Mattila, Pirjo
中科院分区：
文献类型：
--
作者：
Hellstrom, Jarkko;Sinkkonen, Jari;Mattila, Pirjo
采用凝胶吸附和正相液相色谱法从萨斯卡通（Amelanchier alnifolia）果实中分离得到不同聚合度的原花青素低聚物。原花青素的鉴定采用电喷雾质谱，核磁共振光谱，和硫解降解与反相液相色谱法相结合。结果表明，萨斯卡通浆果含有原花青素从二聚体到七聚体和更高的聚合物。萨斯卡通原花青素基本上是原花青素类型，主要由通过B型键连接的表儿茶素单元组成。萨斯卡通浆果的简单原花青素谱允许原花青素根据其聚合度精确分离。这些化合物可作为标准化合物用于原花青素的定性和定量分析以及原花青素生物活性的研究。
Proanthocyanidin oligomers with different degrees of polymerization were isolated from Saskatoon berries (Amelanchier alnifolia) by means of gel adsorption and normal-phase liquid chromatography. The proanthocyanidins were identified using electrospray ionization mass spectrometry, nuclear magnetic resonance spectroscopy, and thiolytic degradation coupled with reversed-phase liquid chromatography. The results established that Saskatoon berries contain proanthocyanidins from dimers through heptamers and higher polymers. Saskatoon proanthocyanidins are essentially of procyanidin type, consisting mainly of epicatechin units linked by B-type bonds. The simple procyanidin profile of Saskatoon berries allowed the procyanidins to be separated precisely according to their degrees of polymerization. In the future they can be used as standard compounds for qualitative and quantitative analysis of procyanidins as well as for elucidation of the biological activities of proanthocyanidins.