Synthesis of nitric oxide in human osteoblasts in response to physiologic stimulation of electrotherapy.
Synthesis of nitric oxide in human osteoblasts in response to physiologic stimulation of electrotherapy.
复制标题
人成骨细胞响应电疗的生理刺激而合成一氧化氮。
DOI：
10.1007/s10439-006-9206-5
复制
发表时间：
2006
影响因子：
3.8
通讯作者：
Cho,Michael
中科院分区：
文献类型：
--
作者：
Hamed,Ayman;Kim,Paul;Cho,Michael
Electrotherapy for bone healing, remodeling and wound healing may be mediated by modulation of nitric oxide (NO). Using NO-specific fluorophore (DAF-2), we report here that application of non-invasive, physiologic electrical stimulation induces NO synthesis in human osteoblasts, and that such NO generation is comparable to that induced by estrogen treatment. For example, application of a sinusoidal 1 Hz, 2 V/cm (peak to peak) electrical stimulation (ES) increases NO-bound DAF-2 fluorescence intensity by a 2-fold within 60 min exposure by activating nitric oxide synthase (NOS). Increase in the NO level is found to depend critically on the frequency and strength of ES. While the frequency of 1 Hz ES seems optimal, the ES strength >0.5 V/cm is required to induce significant NO increase, however. Nitric oxide synthesis in response to ES is completely prevented by blocking estrogen receptors using a competitive inhibitor, suggesting that NO generation is likely initiated by activation of estrogen receptors at the cell surface. Based on these findings, physiologic stimulation of electrotherapy appears to represent a potential non-invasive, non-genomic, and novel physical technique that could be used to regulate NO-mediated bone density and facilitate bone remodeling without adverse effects associated with hormone therapy.
