EXCITATORY AMINO-ACIDS INTERFERE WITH NORMAL EYE GROWTH IN POSTHATCH CHICK
EXCITATORY AMINO-ACIDS INTERFERE WITH NORMAL EYE GROWTH IN POSTHATCH CHICK
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DOI：
10.3109/02713688909000868
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发表时间：
1989-08-01
影响因子：
2
通讯作者：
EHRLICH, D
中科院分区：
文献类型：
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作者：
BARRINGTON, M;SATTAYASAI, J;EHRLICH, D
本研究探讨兴奋性毒性氨基酸对眼睛生长和视网膜形态的影响。一日龄的小鸡接受200纳摩尔红藻氨酸（KA）、200纳摩尔使君子酸（QUIS）或400纳摩尔N-甲基- D，L-天冬氨酸（NMDA）的单次眼内注射。在7、14和21天的存活期后，取出眼球并称重。测量眼轴长度、赤道长度、前房深度和角膜直径。KA治疗增加了眼重和赤道长度。QUIS治疗增加了前房深度，但减少了赤道长度。NMDA治疗增加前房深度，但程度低于QUIS。QUIS和NMDA的作用与KA不同，前者可使前房深度明显增加，而不使玻璃体房扩大。眼睛大小的变化在第7天是明显的，并在整个实验期间持续。视网膜检查显示KA损害无长突细胞、双极细胞、部分节细胞和光感受器。暴露于QUIS会损害无长突细胞、水平细胞，并导致感光细胞外节轻度破坏。相比之下，NMDA主要损害无长突细胞。结果表明，这些神经毒素对眼睛生长有不同的影响，这可能与视网膜病理学的差异有关。有人提出，光感受器非常适合在控制眼睛生长中发挥作用。
This study examines the effects of excitotoxic amino acids on eye growth and retinal morphology. Day old chicks received a single intraocular injection of either 200 nmoles kainic acid (KA), 200 nmoles quisqualic acid (QUIS) or 400 nmoles N-methyl- D,L-aspartate (NMDA). Following survival periods of 7, 14 and 21 days, eyeballs were removed and weighed. Measurements of axial length, equatorial length, anterior chamber depth and corneal diameter were taken. Treatment with KA increased eye weight and equatorial length. Treatment with QUIS increased the anterior chamber depth but decreased the equatorial length. Treatment with NMDA increased anterior chamber depth, but to a lesser extent than QUIS. THe effects of QUIS and NMDA could be distinguished from those of KA since the former excitotoxins resulted in a marked increase in anterior chamber depth with no enlargement of vitreal chamber. Changes in eye size were evident by day 7 and were sustained throughout the duration of the experiment. Examination of retinae revealed that KA lesions amacrine cells, bipolar cells, some ganglion cells and photoreceptors. Exposure to QUIS lesions amacrine cells, horizontal cells and causes mild disruption of photoreceptor outer segments. In contrast, NMDA predominantly lesions amacrine cells. The results demonstrate that these neurotoxins have different effects on eye growth, which may be associated with differences in retial pathology. It is proposed that photoreceptors are ideally suited to play a role in the control of eye growth.