Trypanosoma congolense: In Vitro Culture and Transfection.
Trypanosoma congolense: In Vitro Culture and Transfection.
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DOI：
10.1002/cpmc.77
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发表时间：
2019-06-01
影响因子：
--
通讯作者：
Gibson, Wendy
中科院分区：
文献类型：
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作者：
Kay, Chris;Peacock, Lori;Gibson, Wendy
刚果锥虫（Trypanosoma congolense）与T.间日疟原虫和T.布鲁氏菌引起非洲动物锥虫病（AAT），或nagana，这是一种由撒哈拉以南非洲的吸血采采蝇传播的牲畜疾病。这些寄生原生生物在两个宿主之间循环：哺乳动物和采采蝇。每种宿主提供给锥虫的环境是明显不同的，因此在哺乳动物中发现的阶段的代谢与昆虫阶段的代谢不同。对于新的诊断和治疗方法的研究，使用哺乳动物生命周期阶段，血流形式是合适的。昆虫阶段，如顺周期是有用的研究分化，也作为一个方便的来源，易于培养，非感染性的生物。在这里，我们目前使用的协议在我们的实验室在体外培养的不同生命周期阶段的T。刚果人-顺环体、外鞭毛体和血流形式-以及使生物体能够被遗传修饰的转染方法。John Wiley & Sons，Inc._关于我们
Trypanosoma congolense, together with T. vivax and T. brucei, causes African animal trypanosomiasis (AAT), or nagana, a livestock disease carried by bloodsucking tsetse flies in sub-Saharan Africa. These parasitic protists cycle between two hosts: mammal and tsetse fly. The environment offered by each host to the trypanosome is markedly different, and hence the metabolism of stages found in the mammal differs from that of insect stages. For research on new diagnostics and therapeutics, it is appropriate to use the mammalian life cycle stage, bloodstream forms. Insect stages such as procyclics are useful for studying differentiation and also serve as a convenient source of easily cultured, non-infective organisms. Here, we present protocols in current use in our laboratory for the in vitro culture of different life cycle stages of T. congolense-procyclics, epimastigotes, and bloodstream forms-together with methods for transfection enabling the organism to be genetically modified. © 2019 by John Wiley & Sons, Inc.