Wolbachia: Intracellular manipulators of mite reproduction
Wolbachia: Intracellular manipulators of mite reproduction
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DOI：
10.1007/bf00053306
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发表时间：
1996-08-01
影响因子：
2.2
通讯作者：
Jacobs, G
中科院分区：
文献类型：
--
作者：
Breeuwer, JAJ;Jacobs, G
细胞质传播的沃尔巴克氏菌(α-变形杆菌)是一组密切相关的细胞内微生物，可以改变节肢动物的生殖。它们在少数等足类动物中被发现，并在昆虫中广泛存在。沃尔巴克氏菌与寄生蜂的孤雌生殖、等足目的雌性化和许多昆虫的生殖(细胞质)不亲和性有关。在这里，我们报道了在蜘蛛和捕食性螨类中广泛存在的沃尔巴克氏菌，基于针对沃尔巴克氏菌的特异性引物对FtsZ基因730bp片段的聚合酶链式反应(PCR)。另外一项用两对引物扩增FtsZ基因259个碱基区的聚合酶链式反应(PCR)显示，受感染的螨只携带B型，而不携带A型沃尔巴克氏菌。一些物种的沃尔巴克氏菌检测呈阴性，并不意味着整个物种都没有感染。我们发现二斑叶螨的自然种群对这种感染是多态的。讨论了沃尔巴克氏菌对螨类繁殖和合子后生殖隔离的可能影响。
Cytoplasmically transmitted Wolbachia (alpha-Proteobacteria) are a group of closely related intracellular microorganisms that alter reproduction in arthropods. They are found in a few isopods and are widespread in insects. Wolbachia are implicated as the cause of parthenogenesis in parasitic wasps, feminization in isopods and reproductive (cytoplasmic) incompatibility in many insects. Here we report on the widespread occurrence of Wolbachia in spider mites and predatory mites based on a PCR assay for a 730 bp fragment of the ftsZ gene with primers that are specific for Wolbachia. An additional PCR, using two primer pairs that amplify a 259 bp region of the ftsZ gene that are diagnostic for the two Wolbachia subdivisions A and B, showed that infected mites only carried type B and not type A Wolbachia. The fact that some species tested negative for Wolbachia does not mean that the entire species is uninfected. We found that natural populations of Tetranychus urticae are polymorphic for the infection. The possible effects of Wolbachia on mite reproduction and post-zygotic reproductive isolation are discussed.