Recovery of E,E-Farnesol from Cultures of Yeast erg9 Mutants: Extraction with Polymeric Beads and Purification by Normal-Phase Chromatography
Recovery of E,E-Farnesol from Cultures of Yeast erg9 Mutants: Extraction with Polymeric Beads and Purification by Normal-Phase Chromatography
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DOI：
10.1002/btpr.180
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发表时间：
2009-07-01
影响因子：
2.9
通讯作者：
Song, Linsheng
中科院分区：
文献类型：
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作者：
Song, Linsheng
角鲨烯合成酶阻断的酿酒酵母erg9突变株生长需要麦角甾醇，并产生E，E-法尼醇。通常，至少有一半的法尼醇与细胞相关。法尼醇几乎不溶于水，可以用甲醇/正己烷或聚(苯乙烯-二乙烯基苯)珠从erg9突变株的生产培养物中提取。第一种方法消耗更多的溶剂，并且需要离心法来清除界面乳状液。第二种方法使用的溶剂少50%，珠子可以重复使用提取。微珠提取的无溶剂粗提物的法尼醇含量(76-77%)高于溶剂提取的粗提物(61-65%)。正相色谱分离得到的金合欢醇总收率为94%，纯度为99%。(C)2009年美国化学工程师协会生物技术研究所。计划，25：1111-1114,2009
Saccharomyces cerevisiae erg9 mutants blocked at squalene synthase require ergosterol for growth and produce E,E-farnesol. Typically, at least half the amount of farnesol remains cell associated. Practically insoluble in water, farnesol can be extracted from production cultures of the erg9 mutants using either methanol/hexane or poly(styrene-co-divinylbenzene) beads. The first method consumes more solvents and requires centrifugation to clear an interface emulsion. The second method uses 50% less solvent and the beads can be used repeatedly for extraction. The solvent-free crude extract from the beads extraction contained higher concentration of farnesol (76-77%) than that from the solvent extraction (61-65%). Farnesol was obtained after normal-phase chromatography in high overall recovery (94%) and purity (99%). (C) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 25: 1111-1114, 2009