Impact of SO2 on Arabidopsis thaliana transcriptome in wildtype and sulfite oxidase knockout plants analyzed by RNA deep sequencing
Impact of SO2 on Arabidopsis thaliana transcriptome in wildtype and sulfite oxidase knockout plants analyzed by RNA deep sequencing
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DOI：
10.1111/j.1469-8137.2012.04331.x
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发表时间：
2012-12-01
期刊：
影响因子：
9.4
通讯作者：
Haensch, Robert
中科院分区：
文献类型：
--
作者：
Hamisch, Domenica;Randewig, Doerte;Haensch, Robert
高浓度的二氧化硫 (SO2) 作为一种空气污染物，及其衍生物亚硫酸盐会引起非生物应激，从而导致细胞死亡。目前尚不清楚植物熏蒸在多大程度上触发特定的转录反应。为了解决这个问题，并检验亚硫酸盐氧化酶 (SO) 在 SO2 解毒中发挥作用的假设，我们比较了拟南芥野生型 (WT) 和 SO 敲除品系 (SO-KO) 面临 600 nl l (1) SO2 的影响，使用 RNAseq 量化绝对转录本丰度。这些转录组数据与先前研究中从相同样品中获得的硫代谢相关酶活性和代谢物相关。SO-KO植物在mRNA水平上表现出显着且广泛的调节反应，特别是在与硫代谢酶相关的转录本中，而且在与胁迫反应和衰老相关的转录本中也表现出显着且广泛的调节反应。关注 SO 调节，在 WT 中未检测到任何改变，而在 SO-KO 植物中，我们发现 SO 基因的两个剪接变体上调，尽管该基因在该品系中不起作用。我们的数据为 SO 和硫相关酶（如 APS 还原酶）之间的高度特异性共调节提供了证据，并提出了参与 SO2 解毒的两种新候选物：质外体过氧化物酶、 和防御素作为假定的半胱氨酸大量储存。
High concentrations of sulfur dioxide (SO2) as an air pollutant, and its derivative sulfite, cause abiotic stress that can lead to cell death. It is currently unknown to what extent plant fumigation triggers specific transcriptional responses.To address this question, and to test the hypothesis that sulfite oxidase (SO) is acting in SO2 detoxification, we compared Arabidopsis wildtype (WT) and SO knockout lines (SO-KO) facing the impact of 600 nl l (1) SO2, using RNAseq to quantify absolute transcript abundances. These transcriptome data were correlated to sulfur metabolism-related enzyme activities and metabolites obtained from identical samples in a previous study.SO-KO plants exhibited remarkable and broad regulative responses at the mRNA level, especially in transcripts related to sulfur metabolism enzymes, but also in those related to stress response and senescence. Focusing on SO regulation, no alterations were detectable in the WT, whereas in SO-KO plants we found up-regulation of two splice variants of the SO gene, although this gene is not functional in this line.Our data provide evidence for the highly specific coregulation between SO and sulfur-related enzymes like APS reductase, and suggest two novel candidates for involvement in SO2 detoxification: an apoplastic peroxidase, and defensins as putative cysteine mass storages.