The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria
The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria
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DOI：
10.1038/emboj.2009.36
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发表时间：
2009-04-08
期刊：
影响因子：
11.4
通讯作者：
Rossmann, Michael G.
中科院分区：
文献类型：
--
作者：
Aksyuk, Anastasia A.;Leiman, Petr G.;Rossmann, Michael G.
T4噬菌体的收缩尾巴是一种分子机器，有助于极高的病毒感染效率。它的主要组成部分是尾鞘，在感染期间收缩到不到初始长度的一半。鞘由138个拷贝的尾鞘蛋白基因产物(GP)18组成，包裹着中央非收缩尾管。鞘的收缩驱动尾管穿过外膜，为病毒基因组的传递创造了一个通道。大约四分之三的gp18的晶体结构已经被确定，并被适合于收缩前后的尾鞘的冷冻电子显微镜重建。结果表明，在收缩过程中，gp18亚基相互滑动，结构没有明显变化。
The contractile tail of bacteriophage T4 is a molecular machine that facilitates very high viral infection efficiency. Its major component is a tail sheath, which contracts during infection to less than half of its initial length. The sheath consists of 138 copies of the tail sheath protein, gene product (gp) 18, which surrounds the central noncontractile tail tube. The contraction of the sheath drives the tail tube through the outer membrane, creating a channel for the viral genome delivery. A crystal structure of about three quarters of gp18 has been determined and was fitted into cryo-electron microscopy reconstructions of the tail sheath before and after contraction. It was shown that during contraction, gp18 subunits slide over each other with no apparent change in their structure.