Effects of anodic titanium dioxide nanotubes of different diameters on macrophage secretion and expression of cytokines and chemokines
Effects of anodic titanium dioxide nanotubes of different diameters on macrophage secretion and expression of cytokines and chemokines
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DOI：
10.1111/cpr.12149
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发表时间：
2015-02-01
影响因子：
8.5
通讯作者：
Zhang, Z. T.
中科院分区：
文献类型：
--
作者：
Lu, W. L.;Wang, N.;Zhang, Z. T.
ObjectivesTo investigate effects of TiO2 nanotubes of different diameters on J744A.1 macrophage behaviour, secretion and expression of pro-inflammatory cytokines and chemokines.Materials and methodsMacrophage-like J744A.1 cells were cultured on three types of Ti surface: mechanically polished titanium plus 30 and 80nm TiO2 nanotube surfaces, for 4, 24 and 48h. Macrophage adhesion and proliferation were assessed using CCK-8 assay. Levels of pro-inflammatory cytokines (TNF-, IL-1 and IL-6) and chemokines (MCP-1 and MIP-1) secreted into the supernatant were measured using the Cytometric Bead Arrays kit. TNF-, MCP-1 and MIP-1 gene expression were quantitatively analysed by real-time PCR.ResultsThese show that TiO2 nanotube surfaces, especially of 80nm TiO2 nanotube, benefited macrophage adhesion and proliferation, and reduced protein secretion and mRNA expression of TNF-, MCP-1 and MIP-1. IL-1 and IL-6 were undetectable on all the surfaces at all times.ConclusionsTiO(2) nanotube surfaces, especially of 80nm TiO2 nanotube, reduced inflammatory response in vitro, which might be part of a basis for rapid osseointegration in implants with TiO2 nanotube surfaces in animal studies.
