A study of factors involved in induction of the acrosomal reaction in sperm of the sea urchin, Arbacia punctulata.
A study of factors involved in induction of the acrosomal reaction in sperm of the sea urchin, Arbacia punctulata.
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海胆 Arbacia punctulata 精子顶体反应诱导因素的研究。
DOI：
10.1016/0012-1606(76)90213-x
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发表时间：
1976
影响因子：
2.7
通讯作者：
W. J. Lennarz
中科院分区：
文献类型：
--
作者：
Glenn L. Decker;David B. Joseph;W. J. Lennarz
用一种简单的底物膜技术在电子显微镜下监测顶体突起的延伸，定量地研究了诱导海胆精子顶体反应的几个因素。用薄片验证了典型顶体突起的形成。在无卵冻膜的人工海水中，精子在pH值高于9.6的条件下发生顶体反应。在卵冻存在的情况下，高比例的精子在pH值为8.6时发生反应。在这个pH值下，精子顶体反应的比例与卵冻的浓度成正比。钙离子载体A23187在不含蛋冻的情况下，在pH为8.6的条件下可诱导顶体反应。产生反应的精子比例取决于离子载体的浓度和介质中的钙离子浓度。低水平的La~(3+)离子可以抑制精子顶体反应，而低水平的La~(3+)离子是一种钙离子拮抗剂。这些发现表明，海胆精子的顶体反应与其他细胞类型中依赖于膜融合的分泌过程有显著的相似之处。
Several factors involved in induction of the acrosomal reaction in sperm of the sea urchin,Arbacia punctulata, have been investigated quantitatively using a simple substrate film technique to monitor extension of the acrosomal process by electron microscopy. Verification of typical acrosomal process formation has been accomplished using thin sections. Sperm were found to undergo the acrosomal reaction in artificial sea water in the absence of egg jelly coat at pH values above 9.6. In the presence of egg jelly a high percentage of sperm react at pH 8.6. At this pH, the fraction of sperm that undergo the acrosomal reaction is directly proportional to the concentration of egg jelly. The Ca2+ionophore A23187 induces the acrosomal reaction in the absence of egg jelly at pH 8.6. The proportion of sperm that react is dependent on the concentration of ionophore and on the concentration of Ca2+in the medium. Pretreatment of sperm with low levels of La3+ion, which is known to be a Ca2+ion antagonist, results in inhibition of egg jelly induction of the acrosomal reaction. These findings suggest that there are marked similarities between the acrosomal reaction in sea urchin sperm and membrane fusion dependent secretory processes in other cell types.