Morphometric evaluation of changes with time in optic disc structure and thickness of retinal nerve fibre layer in chronic ocular hypertensive monkeys
Morphometric evaluation of changes with time in optic disc structure and thickness of retinal nerve fibre layer in chronic ocular hypertensive monkeys
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DOI：
10.1016/j.exer.2005.08.001
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发表时间：
2006-03-01
影响因子：
3.4
通讯作者：
Araie, M
中科院分区：
文献类型：
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作者：
Shimazawa, M;Tomita, G;Araie, M
我们研究了通过扫描激光检眼镜（海德堡视网膜断层扫描仪，HRT）在高眼压（实验性青光眼）猴的视盘结构的变化的时间过程中，并阐明了组织学RNFL厚度和HRT参数之间的关系。此外，在视网膜神经纤维层（RNFL）厚度的变化在个别眼睛的时间过程中使用的扫描激光偏振仪与固定的角膜偏振补偿器（GDx FCC）进行了测量。在本研究中，进行了两个单独的实验。在11只食蟹猴的左眼中通过激光小梁成形术诱导慢性眼内压（IOP）升高。在实验1中，在5只猴子的10只眼睛中，在激光治疗12周后测量HRT和GDx参数。在实验2中，在HRT和GDx参数的变化的时间过程中进行了检查之前和1，3，4，5，6，8，10，12，14，16周后，激光治疗在6只猴子的12只眼睛。从GDx获得的延迟值（厚度参数）用于推导上级、内部、鼻和颞象限的厚度和比率参数。比率参数表示为上级和下级象限与鼻象限的比率。在实验1和2中，左眼（高血压）的IOP在整个观察期间持续升高。在实验1的HRT测量中，激光治疗12周后，高血压眼和对照眼之间的8个全局地形参数（除椎间盘面积外）中有7个存在统计学差异。在实验2中，HRT参数以时间依赖性的方式变化，但它们中的每一个几乎在激光治疗后约4周达到平台。在实验1和2中，11只猴子的21只眼睛中，在距视盘边缘1.5个椎间盘直径处的组织学平均RNFL厚度与HRT参数之间存在显著相关性。在实验1中，在个体对照眼（n = 5）中，GDx比率参数与组织学RNFL厚度之间存在良好的相关性。在实验2（n = 6）的实验性青光眼左眼中，GDx比值参数随眼压升高呈时间依赖性下降，表明猴眼在眼压升高的同时，HRT和GDx的视盘地形图和RNFL厚度也随时间变化。HRT（边缘和杯）参数与组织学RNFL厚度显示出良好的相关性，并显着相关性。(c)2005爱思唯尔有限公司保留所有权利。
We examined the time course of changes in optic disc structure by means of a scanning laser ophthalmoscope (Heidelberg Retina Tomograph, HRT) in ocular hypertensive (experimental glaucoma) monkeys, and clarified the relationships between the histological RNFL thickness and HRT parameters. Further, the time course of changes in retinal nerve fiber layer (RNFL) thickness in individual eyes was measured using a scanning laser polarimeter with fixed corneal polarization compensator (GDx FCC). In the present study, two separate experiments were carried out. A chronic intraocular pressure (IOP) elevation was induced by laser trabeculoplasty in the left eye in 11 cynomolgus monkeys. In Experiment 1, the HRT and GDx parameters were measured 12 weeks after the laser treatment in 10 eyes in five monkeys. In Experiment 2, the time course of changes in the HRT and GDx parameters was examined before and 1, 3, 4, 5, 6, 8, 10, 12, 14, and 16 weeks after the laser treatment in 12 eyes in six monkeys. The retardation values (thickness parameters) obtained from the GDx were used to derive thickness and ratio parameters in the superior, interior, nasal and temporal quadrants. Ratio parameters were expressed as a ratio of superior and inferior quadrant to nasal quadrant. After the last measurements, each eye was enucleated, and retinal cross sections were prepared for histological analysis.In the left (hypertensive) eyes, IOP was persistently elevated throughout the observation periods in both Experiments 1 and 2. In the HRT measurements in Experiment 1, seven out of eight global topographic parameters (exception, disc area) were statistically different between the hypertensive and control eyes 12 weeks after the laser treatment. In Experiment 2, the HRT parameters changed in a time-dependent manner, but each of them almost plateaued at about 4 weeks after the laser treatment. Significant correlations were seen between the histological mean RNFL thickness at 1.5 disc diameters from the optic disc margin and the HRT parameters in 21 eyes from 11 monkeys in Experiments 1 and 2. Especially good correlations with histological mean RNFL thickness were seen for the rim volume and cup volume.In Experiment 1, good correlations were found between GDx ratio parameters and histological RNFL thickness in individual fight control eyes (n = 5). In individual left experimental glaucoma eyes of Experiment 2 (n = 6), GDx ratio parameters declined in a time-dependent manner alongside the IOP elevation.In conclusion, alongside the IOP elevation, time-related changes in optic disc topography and RNFL thickness were demonstrated in monkey eyes using HRT and GDx. HRT (rim and cup) parameters showed good correlations with histological RNFL thickness, and significant interrelations. (c) 2005 Elsevier Ltd. All rights reserved.