SEQUENCE COMPARISON IN THE CROSSOVER REGION OF AN ONCOGENIC AVIAN RETROVIRUS RECOMBINANT AND ITS NON-ONCOGENIC PARENT - GENETIC REGIONS THAT CONTROL GROWTH-RATE AND ONCOGENIC POTENTIAL
SEQUENCE COMPARISON IN THE CROSSOVER REGION OF AN ONCOGENIC AVIAN RETROVIRUS RECOMBINANT AND ITS NON-ONCOGENIC PARENT - GENETIC REGIONS THAT CONTROL GROWTH-RATE AND ONCOGENIC POTENTIAL
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DOI：
10.1128/mcb.2.11.1331
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发表时间：
1982-01-01
影响因子：
5.3
通讯作者：
SKALKA, AM
中科院分区：
文献类型：
--
作者：
TSICHLIS, PN;DONEHOWER, L;SKALKA, AM
NTRE 7是内源性非致癌性PrRSV相关病毒0（RAV-0）和劳斯肉瘤病毒B（td-PrRSV-B）的致癌性、外源性、转化缺陷（td）布拉格毒株的禽类逆转录病毒重组体。寡核苷酸图谱显示，重组病毒与其RAV-0亲本无区别，除了3“-末端序列来自td-PrRSV-B。该病毒表现出典型的外源性病毒特性：在组织培养中生长至高滴度，在体内致癌。为了准确地定义负责这些特性的遗传区域，通过使用其DNA的分子克隆来确定重组体及其RAV-0亲本的核苷酸序列。将这些序列与PrRSV-C（一种与外源亲本td-PrRSV-B密切相关的病毒）已有的序列进行比较。结果表明，产生NTRE 7的交换事件发生在从td-PrRSV亲本基因组3“端开始的-501至-401个核苷酸的区域，并且重组区域右侧的序列负责其生长特性和致癌潜力。这些序列包括RAV-0基因组中不存在的148个碱基对的外源病毒特异性区域和长末端重复序列的U3区域。由于预期RSV的Schmidt-Ruppin株的转化缺陷型突变体缺失外源病毒特异性序列，该突变体与其他外源病毒一样，在组织培养物中生长至高滴度，并且在体内致癌，因此得出结论，外源病毒的生长特性和致癌潜力由长末端重复序列的U3区中的序列决定。显然，外源病毒特异性区域可能在决定给定致癌病毒的致癌谱中起作用。
NTRE 7 is an avian retrovirus recombinant of the endogenous nononcogenic Rous-associated virus-0 (RAV-0) and the oncogenic, exogenous, transformation-defective (td) Prague strain of Rous sarcoma virus B (td-PrRSV-B). Oligonucleotide mapping had shown that the recombinant virus is indistinguishable from its RAV-0 parent except for the 3''-end sequences, which were derived from td-PrRSV-B. The virus exhibits properties which are typical of an exogenous virus: it grows to high titers in tissue culture and it is oncogenic in vivo. To accurately define the genetic region responsible for these properties, the nucleotide sequences of the recombinant and its RAV-0 parent were determined by using molecular clones of their DNA. These were compared with sequences already available for PrRSV-C, a virus closely related to the exogenous parent td-PrRSV-B. The results suggested that the crossover event which generated NTRE 7 took place in a region -501 to -401 nucleotides from the 3'' end of the td-PrRSV parental genome and that sequences to the right of the recombination region were responsible for its growth properties and oncogenic potential. These sequences included a 148-base-pair exogenous-virus-specific region that was absent from the RAV-0 genome and the U3 region of the long terminal repeat. Since the exogenous-virus-specific sequences are expected to be missing from transformation-defective mutants of the Schmidt-Ruppin strain of RSV, which, like other exogenous viruses, grow to high titers in tissue culture and are oncogenic in vivo, it was concluded that the growth properties and oncogenic potential of the exogenous viruses are determined by sequences in the U3 region of the long terminal repeat. Evidently, the exogenous-virus-specific region may play a role in determining the oncogenic spectrum of a given oncogenic virus.