γ‐Aminobutyric acidA ρ receptor subunits in the developing rat hippocampus
γ‐Aminobutyric acidA ρ receptor subunits in the developing rat hippocampus
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发育中的大鼠海马中的γ-氨基丁酸A ρ 受体亚基
DOI：
10.1002/jnr.10178
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发表时间：
2002
影响因子：
4.2
通讯作者：
E. Cherubini
中科院分区：
文献类型：
--
作者：
F. Didelon;M. Sciancalepore;N. Savić;M. Mladinić;A. Bradbury;E. Cherubini
采用RT - PCR方法测定了新生大鼠和成年大鼠海马中γ -氨基丁酸(GABA) α ρ受体亚基的表达。所有三个ρ亚基在出生后(P) 2被检测到，ρ3亚基表达在极低水平。ρ1和ρ2产物表现出发育调控；研究发现，这种情况在成年后更为明显。在另一组实验中，为了将基因表达与受体功能联系起来，我们在CA3锥体细胞（来自P3-P4海马切片）中使用单细胞RT - PCR检测GABAA ρ亚基mrna，这些细胞先前通过电生理实验表征了它们对GABA的双管碱敏感或不敏感反应。在19个细胞中，有6个细胞（31%）在100 μM双cuculline（314±129 pA）存在的情况下，GABA施加压力诱发-70 mV内向电流。在其中两个神经元中进行的RT‐PCR显示存在ρ1和ρ2亚基，后者与α2亚基一起存在。在9个神经元中，有1个神经元中存在一个对GABA有反应的ρ2亚基，该亚基被双曲线碱完全消除。这可能是由于缺乏假定的辅助GABAA亚基，这些亚基可以与ρ2结合形成功能性受体。在10个P15 CA3锥体细胞中进行的类似实验没有发现任何ρ1-3转录本。然而，这些神经元大量表达α3亚基。新生儿和成人海马的CA3锥体细胞中可能存在GABAA ρ亚基，但表达水平很低。©2002 Wiley‐Liss, Inc。
The RT‐PCR approach was used to estimate the expression of γ‐aminobutyric acid (GABA)A ρ receptor subunits in the hippocampus of neonatal and adult rats. All three ρ subunits were detected at postnatal day (P) 2, the ρ3 subunit being expressed at an extremely low level. The ρ1 and ρ2 products appeared to be developmentally regulated; they were found to be more pronounced in adulthood. In another set of experiments, to correlate gene expression with receptor function, GABAA ρ subunit mRNAs were detected with single‐cell RT‐PCR in CA3 pyramidal cells (from P3–P4 hippocampal slices), previously characterized with electrophysiological experiments for their bicuculline‐sensitive or ‐insensitive responses to GABA. In 6 of 19 cells (31%), pressure application of GABA evoked at –70 mV inward currents that persisted in the presence of 100 μM bicuculline (314 ± 129 pA). RT‐PCR performed in two of these neurons revealed the presence of ρ1 and ρ2 subunits, the latter being present with the α2 subunit. A ρ2 subunit was also found in 1 neuron (among 9) exhibiting a response to GABA, which was completely abolished by bicuculline. This might be due to the lack of putative accessory GABAA subunits that can coassemble with ρ2 to make functional receptors. Similar experiments from 10 P15 CA3 pyramidal cells failed to reveal any ρ1–3 transcripts. However, these neurons abundantly express α3 subunits. It is likely that in CA3 pyramidal cells of neonatal and adult hippocampus GABAA ρ subunits are present but at very low levels of expression. © 2002 Wiley‐Liss, Inc.
在大鼠视网膜中克隆 γ-氨基丁酸 C 型受体亚基，其蛋氨酸残基对印防己毒素通道阻断至关重要。
DOI：
10.1073/pnas.92.25.11756
发表时间：
1995
影响因子：
11.1
作者：
Zhang,D;Pan,ZH;Zhang,X;Brideau,AD;Lipton,SA
通讯作者：
Lipton,SA
影响因子：
3.6
作者：
D. Ragozzino;Richard M. Woodward;Y. Murata;F. Eusebi;Larry E. Overman;Ricardo Miledi
通讯作者：
D. Ragozzino;Richard M. Woodward;Y. Murata;F. Eusebi;Larry E. Overman;Ricardo Miledi
DOI：
10.1073/pnas.88.10.4318
发表时间：
1991-05-01
影响因子：
11.1
作者：
POLENZANI, L;WOODWARD, RM;MILEDI, R
通讯作者：
MILEDI, R