Identification of Conserved and Novel MicroRNAs during Tail Regeneration in the Mexican Axolotl.
Identification of Conserved and Novel MicroRNAs during Tail Regeneration in the Mexican Axolotl.
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DOI：
10.3390/ijms160922046
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发表时间：
2015-09-11
影响因子：
5.6
通讯作者：
Echeverri K
中科院分区：
文献类型：
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作者：
Gearhart MD;Erickson JR;Walsh A;Echeverri K
墨西哥蝾螈（Ambystoma mexicanum）是一群脊椎动物中的一员，它们保留了惊人的再生身体多个部位的能力。除了作为一个重要的再生模式系统外，美西螈也为基础发育的研究做出了广泛的贡献。虽然许多已知在发育过程中起关键作用的基因现在已经涉及到各种形式的再生，但控制潜在分子电路的许多调节装置仍然未知。近年来，microrna已被确定为发育过程中基因表达的关键调节因子，在许多疾病中，也越来越多地在再生中。在这里，我们使用深度测序结合qRT-PCR对参与调节蝾螈再生的microrna进行了全面鉴定。具体来说，在我们发现的在美西螈组织中表达的microRNA中，我们已经确定了4564个已知在脊椎动物中广泛保守的microRNA家族，以及59,811个推测的新microRNA。这些发现支持了一种假设，即microRNAs在管理基因表达的精确空间和时间模式中发挥关键作用，从而确保缺失组织的正确再生。
The Mexican axolotl salamander (Ambystoma mexicanum) is one member of a select group of vertebrate animals that have retained the amazing ability to regenerate multiple body parts. In addition to being an important model system for regeneration, the axolotl has also contributed extensively to studies of basic development. While many genes known to play key roles during development have now been implicated in various forms of regeneration, much of the regulatory apparatus controlling the underlying molecular circuitry remains unknown. In recent years, microRNAs have been identified as key regulators of gene expression during development, in many diseases and also, increasingly, in regeneration. Here, we have used deep sequencing combined with qRT-PCR to undertake a comprehensive identification of microRNAs involved in regulating regeneration in the axolotl. Specifically, among the microRNAs that we have found to be expressed in axolotl tissues, we have identified 4564 microRNA families known to be widely conserved among vertebrates, as well as 59,811 reads of putative novel microRNAs. These findings support the hypothesis that microRNAs play key roles in managing the precise spatial and temporal patterns of gene expression that ensures the correct regeneration of missing tissues.
DOI：
10.4161/cc.26962
发表时间：
2013-12-15
期刊：
Cell cycle (Georgetown, Tex.)
影响因子：
--
作者：
Crescenzi M
通讯作者：
Crescenzi M