PRESENCE OF A POTENT TRANSCRIPTION ACTIVATING SEQUENCE IN THE P53 PROTEIN
PRESENCE OF A POTENT TRANSCRIPTION ACTIVATING SEQUENCE IN THE P53 PROTEIN
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DOI：
10.1126/science.2144363
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发表时间：
1990-08-31
期刊：
影响因子：
56.9
通讯作者：
JANG, SK
中科院分区：
文献类型：
--
作者：
FIELDS, S;JANG, SK
p53基因在多种人类癌症中经常发生突变。然而，野生型p53基因在生长控制中的作用尚不清楚。含有酵母GAL 4的DNA结合结构域和p53部分的杂合蛋白已被用于显示p53蛋白含有在酵母和哺乳动物细胞中起作用的转录激活序列。p53的NH 2-末端73个残基在哺乳动物细胞中激活转录，与疱疹病毒蛋白VP 16一样有效，其包含已知最强的激活结构域之一。结合先前显示p53定位于细胞核并可与DNA结合的数据，这些结果支持p53的功能之一是激活抑制细胞增殖的基因的转录。
The p53 gene is frequently mutated in a wide variety of human cancers. However, the role of the wild-type p53 gene in growth control is not known. Hybrid proteins that contain the DNA binding domain of yeast GAL4 and portions of p53 have been used to show that the p53 protein contains a transcription-activating sequence that functions in both yeast and mammalian cells. The NH2-terminal 73 residues of p53 activated transcription in mammalian cells as efficiently as the herpes virus protein VP16, which contains one of the strongest known activation domains. Combined with previous data that showed p53 is localized to the nucleus and can bind to DNA, these results support the idea that one function of p53 is to activate the transcription of genes that suppress cell proliferation.