Effect of Period 2 on the proliferation, apoptosis and migration of osteosarcoma cells, and the corresponding mechanisms
Effect of Period 2 on the proliferation, apoptosis and migration of osteosarcoma cells, and the corresponding mechanisms
复制标题
period 2对骨肉瘤细胞增殖、凋亡和迁移的影响及其机制。
DOI：
10.3892/ol.2018.8952
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发表时间：
2018-08-01
期刊：
影响因子：
2.9
通讯作者：
Sun, Yuan-Yuan
中科院分区：
文献类型：
--
作者：
Qin, Tao;Lu, Xiao-Ting;Sun, Yuan-Yuan
周期2(Period 2，PER2)是生物钟的核心基因。在许多类型的人类癌症中已经发现了PER2基因的失调，这可能与预后不良有关。为证实PER2基因对MNNG/HOS人骨肉瘤细胞的影响，将针对PER2基因的小干扰(Si)RNA或含PER2基因的小干扰(Si)RNA导入MNNG/HOS细胞，观察细胞增殖、凋亡及迁移情况。本研究表明，PER2基因敲除可显著促进MNNG/HOS细胞的增殖和迁移，并保护MNNG/HOS细胞免于凋亡。PER2过表达抑制MNNG/HOS细胞的增殖和迁移，促进细胞凋亡。此外，PER2过表达细胞中磷酸化(P)蛋白激酶B(Akt)和Bcl2的蛋白表达受到抑制，而p27、p21和裂解的caspase-3的表达受到促进。而在PER2基因敲除细胞中，p-Akt和Bcl2表达增强，p27、p21和caspase-3表达降低。这项初步研究可能为骨肉瘤的治疗提供另一种治疗策略。
Period 2 (per2) is a core circadian clock gene. Dysregulation of the per2 gene has been identified in a number of types of human cancer and may be associated with a poor prognosis. To confirm the influence of per2 gene on MNNG/HOS human osteosarcoma cells, small interfering (si)RNA against per2 or plasmids containing per2 were transfected into MNNG/HOS cells, and the proliferation, apoptosis and migration were observed. The present study demonstrated that per2 knockdown significantly enhanced MNNG/HOS cell proliferation and migration and protected MNNG/HOS cells from apoptosis. Per2 overexpression inhibited MNNG/HOS cell proliferation and migration and promoted apoptosis. Furthermore, the protein expression of phosphorylated (p)-protein kinase B (Akt) and Bcl-2 were inhibited in per2-overexpressing cells, while the expression of p27, p21 and cleaved caspase-3 was promoted. In contrast, the expression of p-Akt and Bcl-2 was promoted in per2-knockdown cells, and p27, p21 and cleaved caspase-3 were decreased. This initial study may provide an alternative therapeutic strategy for the treatment of osteosarcoma.