Autophagic chemicals effect for male-killing Wolbachia, Atg8 and TOR genes in Ostrinia scapulalis (Lepidoptera:Crambidae)
Autophagic chemicals effect for male-killing Wolbachia, Atg8 and TOR genes in Ostrinia scapulalis (Lepidoptera:Crambidae)
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DOI：
10.1007/s13355-023-00818-9
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发表时间：
2023-03-08
影响因子：
1.3
通讯作者：
Tagami，Yohsuke
中科院分区：
文献类型：
--
作者：
Gazali，Achmad;Sugimoto，Takafumi N.;Tagami，Yohsuke
红小豆螟Ostrinia scapulalis（步行者）感染了雄性沃尔巴克氏体（Wolbachia），其在胚胎和幼虫发育阶段选择性地杀死雄性后代，并允许雌性后代存活至成年。高沃尔巴克氏体密度导致强烈的雄性杀伤效应。本研究利用雷帕霉素和3-甲基腺嘌呤作为自噬诱导剂和抑制剂，通过调控自噬来改变Wolbachiadenosity，观察了雷帕霉素和3-甲基腺嘌呤对O. Atg 8和TOR基因被用来预测自噬活性。实时荧光定量PCR检测Wolbachia、Atg 8和TOR的相对密度和表达量。我们报告说，相对密度和表达的沃尔巴克氏体和TOR雷帕霉素治疗减少，而相对表达的Atg 8的增加，在幼虫和成人的治疗。3-甲基腺嘌呤处理表现出与雷帕霉素相反的效果，确切地说，Wolbachia和TOR的相对密度和表达增加，Atg 8的相对表达降低。幼虫处理中的成虫和成虫处理中的后代的雌性比例不受自噬化学物质的影响。幼虫经雷帕霉素处理后，历期明显延长，体重明显下降。自噬药物处理可增加死亡率。Rap处理组翅的畸形率明显高于正常翅。
The adzuki bean borerOstrinia scapulalis(Walker) is infected with male-killingWolbachia, which selectively kills male offspring during the embryonic and larval development stages and allows the female offspring survive to adulthood. A highWolbachiadensity leads to a strong male-killing effect. We utilized rapamycin and 3-methyladenine as an autophagy inducer and inhibitor to manipulate the autophagy which can changeWolbachiadensity and observed their effects onWolbachiadensity in larvae and adults ofO. scapulalis.Atg8andTORgenes were exploited to predict autophagy activity. The relative density and expression ofWolbachia,Atg8, andTORwere counted by quantitative real-time PCR. We report that the relative density and expression ofWolbachiaandTORwere reduced by rapamycin treatments, whereas the relative expression ofAtg8was increased in both the larval and adult treatments. The 3-methyladenine treatments exhibited an opposite effect to rapamycin, precisely, relative density and expression ofWolbachiaandTORwere increased and relative expression ofAtg8was decreased. The female ratio of adults in the larval treatment and offspring in the adult treatments were not affected by the autophagic chemicals. The larval periods were significantly longer and the body weight decreased when the rapamycin was treated to the larvae. The mortality was increased by autophagic chemicals treatment. The abnormality of wing was observed more than normal wing by Rap treatments.