Evaluation of adherence, hemagglutination, and presence of genes codifying for virulence factors of Acinetobacter baumannii causing urinary tract infection
Evaluation of adherence, hemagglutination, and presence of genes codifying for virulence factors of Acinetobacter baumannii causing urinary tract infection
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DOI：
10.1590/s0074-02762004000800010
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发表时间：
2004-12-01
期刊：
影响因子：
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通讯作者：
Vidotto, Marilda Carlos
中科院分区：
文献类型：
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作者：
Braun, Graziela;Vidotto, Marilda Carlos
鲍曼不动杆菌是一种严格的需氧细菌，可引起严重感染，但其致病特征尚未明确。对不同年龄住院和非住院患者尿液中分离的13株鲍曼不动杆菌进行了毒力因子检测。分离株分别为2、6、9生物型，对亚胺培南敏感。多数对阿米卡星、头孢他啶、头孢曲松、环丙沙星、庆大霉素、诺氟沙星、甲氧苄啶-磺胺甲恶唑耐药。通过聚合酶链反应（PCR）检测，没有一株鲍曼不动杆菌菌株存在先前在尿路致病性大肠杆菌中描述的17种不同毒力因子的编码基因。我在甘露糖存在的情况下分离出凝集的人AB型红细胞，但没有凝集的O型红细胞。在7株分离株中观察到对聚苯乙烯的粘附，这一结果与血凝试验结果不相关。所有分离株均能在限铁条件下生长，表明鲍曼不动杆菌能产生一些成熟的铁氧化物。然而，大肠杆菌和耶尔森氏菌的铁摄取系统中的iutA和fyuA基因在分离株中不存在，表明存在不同类型的铁载体。介导粘附的鲍曼不动杆菌菌株的菌毛可能具有甘露糖抗性，尽管其粘附人上皮细胞的机制仍有待阐明。
Acinetobacter baumannii is a strictly aerobic bacterium which causes severe infections, however its pathogenic characteristics are not well defined. Thirteen A. baumannii strains isolated from urine of hospitalized and nonhospitalized patients with different ages were investigated for the presence of virulence factors. The isolates belonged to biotypes 2, 6, and 9 and were sensitive to imipenem. The majority of them showed resistance to amikacin, ceftazidime, ceftriaxone, ciprofloxacin, gentamicin, norfloxacin, and trimethoprim-sufamethoxazole. None of A. baumannii strains presented genes codifying for 17 different virulence factors previously described in uropathogenic Escherichia coli, when tested by polymerase chain reaction (PCR). Mine isolates agglutinated human,group AB erythrocytes, in presence of mannose, but none of them agglutinated group O erythrocytes. Adherence to polystyrene was observed in 7 isolates, and this result did not correlate with that obtained in hemagglutination assay. All the isolates were able to grow in iron-limiting conditions, showing that A. baumannii produces some ripe of siderphore. However the genes iutA and fyuA, from iron uptake system of E. coli and Yersinia sp., respectively, were not present in the isolates, suggesting the presence of a different type of siderophore. The fimbriae of A. baumannii strains that mediates the adherence are possibly mannose-resistant, eventhough the mechanism of adherence to human epithelial cells still remains to be elucidated.