Assembly of phage lambda in vitro.
Assembly of phage lambda in vitro.
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噬菌体 lambda 的体外组装。
DOI：
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发表时间：
1966
影响因子：
11.1
通讯作者：
J. Weigle
中科院分区：
文献类型：
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作者：
J. Weigle
埃德加和伍德[1]最近已经表明，可以在体外组装噬菌体T4的部分，这些部分在突变感染细胞中积累以形成感染性的活噬菌体颗粒。如下所示，这种体外互补作用也可以在λ噬菌体中得到证实。
人们会认为λ的结构没有T4那么复杂，因为λ的染色体只有T4的四分之一的DNA含量，而且可能包含更少的基因。事实上，发现如体外组装所揭示的，λ基因的生理功能仅分为两组-头供体和尾供体，用埃德加和伍德的术语[1]。证据表明，头和尾的λ可以团结起来，形成可行的噬菌体在没有其他因素。
Edgar and Wood [1] have shown recently that it is possible to assemble, in vitro, the parts of phage T4 which accumulate in mutant infected cells to form infectious, viable phage particles. This complementation in vitro can also be demonstrated for phage lambda, as is shown below.
One would expect lambda to be less complex in structure than T4, since the chromosome of lambda has only one fourth of the DNA content of T4 and presumably contains fewer genes. Indeed, it is found that the physiological functions of the genes of lambda, as revealed by in vitro assemblage, divide into two groups only-head donors and tail donors, in the terminology of Edgar and Wood [1]. Evidence is presented which suggests that the heads and tails of lambda can unite to form viable phage in the absence of other factors.