Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.
Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.
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锥虫中的钙调蛋白基因串联重复并产生具有共同 5 前导序列的多个 mRNA。
DOI：
10.1073/pnas.82.12.3998
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发表时间：
1985
影响因子：
11.1
通讯作者：
Richards,FF
中科院分区：
文献类型：
--
作者：
Tschudi,C;Young,AS;Ruben,L;Patton,CL;Richards,FF
在布氏冈比亚锥虫中，Ca2+ 结合蛋白钙调蛋白由三个相同的串联重复基因编码。这些基因的转录本由几种大小相似的 RNA 组成。 35 个核苷酸的剪接前导序列存在于每个 mRNA 的 5' 末端，但不由与这些基因相邻的 DNA 编码。我们已经确定了前导序列与 mRNA 融合的两个不同位点。这些结果有力地支持了这样的观点：这些寄生虫使用了一种新颖的、可能不连续的转录机制。
In Trypanosoma brucei gambiense, the Ca2+ binding protein calmodulin is encoded by three identical tandemly repeated genes. The transcripts of these genes consist of several RNA species similar in size. A 35-nucleotide spliced leader sequence is present at the 5' end of each mRNA but is not encoded by DNA contiguous to these genes. We have identified two different sites for the fusion of the leader to the mRNA. These results strongly support the idea that a novel, possibly discontinuous, transcription mechanism is used by these parasites.