A Qualitative Phytochemical Analysis and a Comparative Study of the Antibactrial Activity of Retama stalks (raetam)
A Qualitative Phytochemical Analysis and a Comparative Study of the Antibactrial Activity of Retama stalks (raetam)
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DOI：
10.13005/ojc/320135
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发表时间：
2016-03-01
影响因子：
0.5
通讯作者：
Tabchouche, Ahmed
中科院分区：
文献类型：
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作者：
Bensaci, Hamza;Sekhri, Lakhdar;Tabchouche, Ahmed
本研究主要采用孔扩散法研究和比较雷公藤甲醇、乙醚和乙酸乙酯提取物对大肠杆菌、沙门氏菌、奇异变形杆菌和金黄色葡萄球菌的抑菌活性。研究结果表明，除奇异变形杆菌外，对所有细菌均有显著作用。经初步鉴定，其成分中含有树脂、挥发油、香豆素、萜烯、酚类、单宁、生物碱、皂苷、心苷和黄酮等活性物质。甲醇提取物在浓度为100 mg/ml时对沙门氏菌的抑制率最高为16 mm，对大肠杆菌在浓度为1 mg/ml时的抑制率最低为8 mm。本研究的结果表明，扫把茎可用于治疗由试验生物引起的疾病。可以进行进一步的化学和药理学研究以分离和鉴定所选植物中负责抗菌活性的化学成分。
The present work is aimed mainly to investigate and compare the antibacterial activities of methanolic, diethyl ether and ethyl acetate extracts of retama on Escherichia coli, Salmonella, Proteus mirabilis and Staphylococcus aureu using well difusion method. The results of study showed a significant effect on all bacterial species except Proteus mirabilis. The preliminary test of retama constituents revealed the presence of active material : Resins, Volatil oils, Coumarins, Terpenes, Phenols, Tannins, Alkaloids, Saponins, Cardiac glycosides, and Flavons. The highest Inhibition rate of Salmonella is 16 mm at the concentration 100 mg/ml, while the lowest inhibition rate was 8 mm for Escherichia coli at concentration 1 mg/ml in methanolic extract. The results obtained in the present study suggest that the retama stalks (broom broom) can be used in treating deseases caused by the tested organisms. Further chemical and pharmacological investigations may be carried out to isolate and identify the chemical constituents in the selected plants responsible for the antimicrobial activity.