Alginate Sulfate-Nanocellulose Bioinks for Cartilage Bioprinting Applications
Alginate Sulfate-Nanocellulose Bioinks for Cartilage Bioprinting Applications
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DOI：
10.1007/s10439-016-1704-5
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发表时间：
2017-01-01
影响因子：
3.8
通讯作者：
Zenobi-Wong, Marcy
中科院分区：
文献类型：
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作者：
Mueller, Michael;Ozturk, Ece;Zenobi-Wong, Marcy
生物打印的挑战之一是识别支持细胞生长、组织成熟以及最终形成用于再生医学的功能性移植物的生物墨水。然而，这种新的生物织物技术对活细胞生物学的影响仍在评估中。最近，我们已经鉴定了基于藻酸盐硫酸盐的促有丝分裂水凝胶系统，其有效地支持软骨细胞表型，但由于其流变学性质（无屈服点）而不可打印。为了将藻酸盐硫酸盐转化为可印刷的生物油墨，将其与纳米纤维素结合，纳米纤维素已被证明具有非常好的可印刷性。藻酸盐硫酸盐/纳米纤维素墨水显示出良好的打印性能，并且非打印的生物墨水材料促进了包封细胞的细胞铺展、增殖和胶原蛋白II合成。当打印生物墨水时，细胞的生物性能高度依赖于喷嘴的几何形状。在最低的挤出压力和剪切应力下保持细胞铺展特性。然而，挤出藻酸盐硫酸盐/纳米纤维素生物墨水和软骨细胞显著损害细胞增殖，特别是当使用小直径喷嘴和阀时。
One of the challenges of bioprinting is to identify bioinks which support cell growth, tissue maturation, and ultimately the formation of functional grafts for use in regenerative medicine. The influence of this new biofabrication technology on biology of living cells, however, is still being evaluated. Recently we have identified a mitogenic hydrogel system based on alginate sulfate which potently supports chondrocyte phenotype, but is not printable due to its rheological properties (no yield point). To convert alginate sulfate to a printable bioink, it was combined with nanocellulose, which has been shown to possess very good printability. The alginate sulfate/nanocellulose ink showed good printing properties and the non-printed bioink material promoted cell spreading, proliferation, and collagen II synthesis by the encapsulated cells. When the bioink was printed, the biological performance of the cells was highly dependent on the nozzle geometry. Cell spreading properties were maintained with the lowest extrusion pressure and shear stress. However, extruding the alginate sulfate/nanocellulose bioink and chondrocytes significantly compromised cell proliferation, particularly when using small diameter nozzles and valves.