MEASUREMENT OF CELL NUMBERS BY MEANS OF THE ENDOGENOUS ENZYME HEXOSAMINIDASE - APPLICATIONS TO DETECTION OF LYMPHOKINES AND CELL-SURFACE ANTIGENS
MEASUREMENT OF CELL NUMBERS BY MEANS OF THE ENDOGENOUS ENZYME HEXOSAMINIDASE - APPLICATIONS TO DETECTION OF LYMPHOKINES AND CELL-SURFACE ANTIGENS
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DOI：
10.1016/0022-1759(84)90477-0
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发表时间：
1984-01-01
影响因子：
2.2
通讯作者：
LANDEGREN, U
中科院分区：
文献类型：
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作者：
LANDEGREN, U
通过使用显色底物的普遍存在的溶酶体酶，氨基己糖苷酶来估计细胞数量，开发了一种灵敏和简单的程序，其中微量反应威尔斯孔直接在分光光度计中扫描。该方法适用于几种细胞生物学测定[包括以下细胞系：人T白血病Jurkat细胞、伯基特淋巴瘤Raji细胞、小鼠T细胞白血病CTLL-2细胞和人羊膜WISH细胞]。T细胞生长因子和干扰素的生物活性的定量可以在大量样品上进行。分散的实体组织细胞与纤连蛋白包被的基质的粘附可以用很少的试剂消耗来定量。通过在微量滴定板中使用淘选程序，开发了用于单克隆抗体与细胞表面抗原结合的灵敏且非常简单的测定法。
By using a chromogenic substrate for an ubiquitous lysosomal enzyme, hexosaminidase to estimate cell numbers, a sensitive and simple procedure was developed in which microtiter reaction wells are directly scanned in a spectrophotometer. This method was adapted to several cell biological assays [including the following cell lines: human T leukemia Jurkat cells, Burkitts lymphoma Raji cells, mouse T cell leukemia CTLL-2 cells and human amniotic WISH cells]. Quantitation of the biological activities of T cell growth factor and interferon can be performed on large numbers of samples. Adhesion of dispersed solid tissue cells to fibronection coated substrates may be quantitated with little expenditure of reagents. By use of a panning procedure in microtiter plates a sensitive and very simple assay for the binding of monoclonal antibodies to cell surface antigens was developed.