Effect of prostate cancer cell line supernatant on functional polarization in macrophages
Effect of prostate cancer cell line supernatant on functional polarization in macrophages
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DOI：
10.4149/bll_2018_095
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发表时间：
2018-01-01
影响因子：
1.5
通讯作者：
Masarik, M.
中科院分区：
文献类型：
--
作者：
Mazalova, L.;Sladek, Z.;Masarik, M.
目的：研究前列腺癌细胞PC-3上清液对巨噬细胞M1/M2功能极化的影响。背景：细胞因子在癌变过程中起重要作用。它们大多由巨噬细胞产生。巨噬细胞分为M1组和M2组。经典表型巨噬细胞M1支持促炎作用，并产生促炎细胞因子，如白细胞介素6 （IL-6）、白细胞介素12 （IL-12）、肿瘤坏死因子α (tnf - α)。M2型巨噬细胞分泌抗炎细胞因子，如白细胞介素10 （IL-10）、转化生长因子β (tgf - β)。方法：外周血单核细胞培养7天，在此期间分化为巨噬细胞。用脂多糖（LPS）刺激巨噬细胞24小时作为阳性对照，再用上清液培养24小时。结果：以不加LPS和不加上清培养的巨噬细胞为阴性对照。采用实时荧光定量PCR检测IL-6、IL-10、IL-12、tnf - α的相对表达量。与阳性对照相比，巨噬细胞上清中促炎细胞因子的表达较低。结论：巨噬细胞上清（M phi+sup）中促炎细胞因子的表达低于阳性对照（M phi+LPS）。未证实上清液对IL-6、IL-10、IL-12和tnf - α表达的影响（表1，图5，参考文献15）。PDF文本www.elis.sk。
OBJECTIVES: We aimed on effect of supernatant derived from prostate cancer cell line PC-3 on M1/M2 functional polarization in macrophages.BACKGROUND: Cytokines play an important role in carcinogenesis. Most of them are produced by macrophages. Macrophages are divided into groups M1 or M2. Classical phenotype macrophages M1 support pro-inflammatory effects and produce pro-inflammatory cytokines, such as interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor alpha (TNF-alpha). Macrophages exhibiting a phenotype M2 secrete anti-inflammatory cytokines, e.g. interleukin 10 (IL-10), transforming growth factor beta (TGF-beta).METHODS: Peripheral blood monocytes were cultivated for 7 days and during this time went through a differentiation into macrophages. Macrophages were stimulated for 24 hours by lipopolysaccharide (LPS) as a positive control and cultivated with supernatant for another 24 hours.RESULTS: Macrophages cultivated without LPS and without supernatant were used as negative control. Relative expression of IL-6, IL-10, IL-12 and TNF-alpha was measured by Quantitative real-time PCR. Expression of pro-inflammatory cytokines was lower in macrophages with supernatant compared to positive control.CONCLUSION: Expression of pro-inflammatory cytokines was lower in macrophages with supernatant (M phi+sup) compared to positive control (M phi+LPS). Effect of the supernatant on expression of IL-6, IL-10, IL-12 and TNF-alpha was not confirmed (Tab. 1, Fig. 5, Ref. 15). Text in PDF www.elis.sk.