Immunodominant linear B cell epitopes in the spike and membrane proteins of SARS-CoV-2 identified by immunoinformatics prediction and immunoassay.
Immunodominant linear B cell epitopes in the spike and membrane proteins of SARS-CoV-2 identified by immunoinformatics prediction and immunoassay.
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DOI：
10.1038/s41598-021-99642-w
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发表时间：
2021-10-14
影响因子：
4.6
通讯作者：
Roshorm YM
中科院分区：
文献类型：
--
作者：
Polyiam K;Phoolcharoen W;Butkhot N;Srisaowakarn C;Thitithanyanont A;Auewarakul P;Hoonsuwan T;Ruengjitchatchawalya M;Mekvichitsaeng P;Roshorm YM
SARS-CoV-2继续感染越来越多的人，导致全球死亡人数增加。随着新变种的出现，目前可用的疫苗效力也相应下降，突显出需要对疫苗设计和评估的病毒表位特征有更深入的了解。在本研究中，通过免疫信息学预测确定了SARS-CoV-2刺突受体结合域(RBD)中的3个免疫优势线性B细胞表位，并用SARS-CoV-2 RBD亚单位疫苗免疫后的恒河猴血清进行了ELISA验证。通过对这三个表位的进一步免疫信息学分析，提出了线性B细胞表位预测和选择的方法。随后，使用新冠肺炎感染患者的恢复期血清预测并确认了尖峰蛋白(S)、膜蛋白(M)和包膜蛋白(E)中的B细胞表位。免疫优势表位位于S_2结构域的3个区域，1个位于S_1/S_2裂解位点，1个位于M蛋白的C-末端。表位图谱显示，在所关注的变体中发现的大部分氨基酸变化位于NTD、RBD和S1/S2裂解位点的B细胞表位内。这项工作为SARS-CoV-2的B细胞表位以及免疫信息学预测B细胞表位的方法提供了见解，这将改进和加强SARS-CoV-2疫苗的开发，以对抗新出现的变异株。
SARS-CoV-2 continues to infect an ever-expanding number of people, resulting in an increase in the number of deaths globally. With the emergence of new variants, there is a corresponding decrease in the currently available vaccine efficacy, highlighting the need for greater insights into the viral epitope profile for both vaccine design and assessment. In this study, three immunodominant linear B cell epitopes in the SARS-CoV-2 spike receptor-binding domain (RBD) were identified by immunoinformatics prediction, and confirmed by ELISA with sera from Macaca fascicularis vaccinated with a SARS-CoV-2 RBD subunit vaccine. Further immunoinformatics analyses of these three epitopes gave rise to a method of linear B cell epitope prediction and selection. B cell epitopes in the spike (S), membrane (M), and envelope (E) proteins were subsequently predicted and confirmed using convalescent sera from COVID-19 infected patients. Immunodominant epitopes were identified in three regions of the S2 domain, one region at the S1/S2 cleavage site and one region at the C-terminus of the M protein. Epitope mapping revealed that most of the amino acid changes found in variants of concern are located within B cell epitopes in the NTD, RBD, and S1/S2 cleavage site. This work provides insights into B cell epitopes of SARS-CoV-2 as well as immunoinformatics methods for B cell epitope prediction, which will improve and enhance SARS-CoV-2 vaccine development against emergent variants.
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影响因子：
11.8
作者：
Maggi F;Novazzi F;Genoni A;Baj A;Spezia PG;Focosi D;Zago C;Colombo A;Cassani G;Pasciuta R;Tamborini A;Rossi A;Prestia M;Capuano R;Azzi L;Donadini A;Catanoso G;Grossi PA;Maffioli L;Bonelli G
通讯作者：
Bonelli G
影响因子：
3.7
作者：
Cruz DJ;Kim CJ;Shin HJ
通讯作者：
Shin HJ
影响因子：
4.8
作者：
Lon JR;Bai Y;Zhong B;Cai F;Du H
通讯作者：
Du H
影响因子：
3.6
作者：
Kringelum JV;Nielsen M;Padkjær SB;Lund O
通讯作者：
Lund O
影响因子：
2.9
作者：
PARKER, JMR;GUO, D;HODGES, RS
通讯作者：
HODGES, RS