Extracellular dopamine and serotonin after ethanol monitored with 5-minute microdialysis.
Extracellular dopamine and serotonin after ethanol monitored with 5-minute microdialysis.
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通过 5 分钟微透析监测乙醇后的细胞外多巴胺和血清素。
DOI：
10.1016/s0741-8329(99)00006-3
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Yan,QS
中科院分区：
文献类型：
--
作者：
Yan,QS
为了确定潜在的快速变化，细胞外多巴胺（DA）和5-羟色胺（5-羟色胺，5-HT）在第一个5-10分钟后，乙醇，脑内微透析结合微孔HPLC。以0.5、1和2 g/kg的剂量IP给予乙醇，并测定自由活动大鼠延髓核（NAC）5分钟透析液中的细胞外DA和5-HT。乙醇在所有剂量下均显著增加细胞外DA和5-HT。DA和5-HT分别在0.5、1和2 g/kg剂量下10、15、20 min和20、20、55 min达到峰值。基于先前的报告显示脑或血液乙醇水平在给药后10-20分钟内达到峰值，结果表明NAC中细胞外DA和5-HT的峰值增加发生在大致相同的时间，DA时程与文献中报告的血液或脑乙醇浓度曲线在时间上更相关。这些结果支持了乙醇的增强特性至少部分来自其刺激NAC中DA释放的能力的概念。此外，目前的数据并不与5-HT在乙醇强化效应中的作用相反。
In order to determine potential rapid changes in extracellular dopamine (DA) and 5-hydroxytryptamine (serotonin, 5-HT) during the first 5–10 min after ethanol, intracerebral microdialysis coupled with microbore HPLC was used. Ethanol at the doses of 0.5, 1, and 2 g/kg was administered IP and extracellular DA and 5-HT in 5-min dialysates from the nucleus accumbens (NAC) of freely moving rats were determined. Ethanol at all doses significantly increased extracellular DA and 5-HT. DA and 5-HT peaked at 10, 15, 20 min and 20, 20, 55 min following 0.5, 1, and 2 g/kg, respectively. Based on previous reports showing that brain or blood ethanol levels peaked within 10–20 min after administration, the results indicate that peak increases in extracellular DA and 5-HT in the NAC occurred at approximately the same time, with the DA time course more temporally correlated with the blood or brain alcohol concentration curve reported in the literature. These results support the concept that the reinforcing properties of ethanol derive, at least in part, from its ability to stimulate DA release in the NAC. In addition, the present data are not in opposition with a role of 5-HT in the ethanol reinforcing effect.