Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles
Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles
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DOI：
10.1093/molehr/gan044
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发表时间：
2008-09-01
影响因子：
4
通讯作者：
Engel, Wolfgang
中科院分区：
文献类型：
--
作者：
Zovoilis, Athanasios;Nolte, Jessica;Engel, Wolfgang
从成年小鼠睾丸分离并培养的精原干细胞（SSCs）已显示出对培养条件的响应并成为多能性的，所谓的多能成体生殖系干细胞（maGSCs）。属于290和302 miRNA簇的microRNA（miRNA）先前已被分类为胚胎干细胞（ESC）特异性的。在这里，我们表明这些miRNA通常表征多能细胞。它们不仅在ESC中表达，而且在maGSC以及F9胚胎癌细胞（ECC）系中表达。此外，我们测试了促进多能性丧失的不同因素对所有三种多能细胞类型中这些miRNA水平的时间依赖性影响。尽管在ESC和maGSC之间观察到分化的时间和程度存在差异，但两种miRNA家族的表达谱显示这两种细胞类型之间的相似性，表明在维持多能性和分化方面存在相似的潜在机制。我们的研究结果表明，290-miRNA家族与Oct-4和多能状态的维持有关。相反，302-miRNA家族的成员在所有测试的细胞类型中在体外分化的第一阶段被诱导。因此，在多能细胞中检测到miR-302家族的miRNA可归因于多能细胞培养物中自发分化细胞的比例。这些结果与maGSC的类胚胎干细胞性质及其作为非胚胎组织多能细胞替代来源的潜力一致。
Spermatogonial stem cells (SSCs) isolated from the adult mouse testis and cultured have been shown to respond to culture conditions and become pluripotent, so called multipotent adult germline stem cells (maGSCs). microRNAs (miRNAs) belonging to the 290 and 302 miRNA clusters have been previously classified as embryonic stem cell (ESC) specific. Here, we show that these miRNAs generally characterize pluripotent cells. They are expressed not only in ESCs but also in maGSCs as well as in the F9 embryonic carcinoma cell (ECC) line. In addition, we tested the time-dependent influence of different factors that promote loss of pluripotency on levels of these miRNAs in all three pluripotent cell types. Despite the differences regarding time and extent of differentiation observed between ESCs and maGSCs, expression profiles of both miRNA families showed similarities between these two cell types, suggesting similar underlying mechanisms in maintenance of pluripotency and differentiation. Our results indicate that the 290-miRNA family is connected with Oct-4 and maintenance of the pluripotent state. In contrast, members of the 302-miRNA family are induced during first stages of in vitro differentiation in all cell types tested. Therefore, detection of miRNAs of miR-302 family in pluripotent cells can be attributed to the proportion of spontaneously differentiating cells in cultures of pluripotent cells. These results are consistent with ESC-like nature of maGSCs and their potential as an alternative source of pluripotent cells from non-embryonic tissues.