Three-dimensional representation and quantification of endosomes in the rat kidney proximal tubule cell.
Three-dimensional representation and quantification of endosomes in the rat kidney proximal tubule cell.
复制标题
大鼠肾近曲小管细胞内体的三维表征和定量。
DOI：
--
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
T. Hatae
中科院分区：
文献类型：
--
作者：
Y. Kawai;T. Hatae
在连续切片示踪的基础上，采用计算机辅助三维重建方法研究了大鼠肾近端小管细胞内体腔室的组织结构。除了众所周知的小泡、小管和大液泡等内吞成分外，内体室还包含一个突出的结构复合体，由中央液泡成分（直径可达2000纳米）和相关的辐射管状结构组成。所检查的大核内体有2至24个从中央液泡随机指向的直或弯曲延伸小管。这些小管直径在70 ~ 90 nm之间，随长度的变化而变化，最长可达1500 nm。从具有10个以上管状延伸的大核内体的例子中进行定量评价，表明25-40%的膜位于管状部分，而体积的5-10%。这些结果表明，内体小管作为膜再循环介质的高效作用，因为在再循环过程中，小管同时允许在中央液泡中保留大部分内容物。
The organization of the endosomal compartment in the rat kidney proximal tubule cell was studied by computer-aided three-dimensional reconstruction based on tracing of serial thin sections. Besides the well-documented endocytic elements such as small vesicles, tubules and large vacuoles, the endosomal compartment contained a prominent structural complex consisting of a central vacuolar element (with diameters up to 2000 nm) and associated systems of radiating tubular profiles. The large endosomes examined had from two to twenty-four tubules as straight or bending extensions directed randomly from the central vacuole. The tubules were 70-90 nm in diameter and varied with the length up to 1500 nm. Quantitative evaluation from examples of the large endosomes with more than ten tubular extensions, showed that 25-40% of the membrane resided in the tubular portion in contrast to 5-10% of the volume. These results indicate the high efficiency of the role of the endosomal tubules as a mediator of the membrane recycling, because the tubules simultaneously allow retention of most of the content in the central vacuole during the recycling process.