Antiterminator protein GlpP of Bacillus subtilis binds to glpD leader mRNA
Antiterminator protein GlpP of Bacillus subtilis binds to glpD leader mRNA
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DOI：
10.1099/00221287-144-2-449
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发表时间：
1998-02-01
期刊：
影响因子：
--
通讯作者：
Rutberg, B
中科院分区：
文献类型：
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作者：
Glatz, E;Persson, M;Rutberg, B
枯草杆菌glpD基因编码3-磷酸甘油(G3P)脱氢酶。GlpD的表达主要受glpD前导序列中反向重复序列转录终止/反终止的控制。在G3P存在的情况下，抗终止是由抗终止蛋白GlpP介导的。本文报道了GlpP与glpD先导基因在体内和体外的相互作用。在体内，GlpP的抗终止作用可以在携带GlpD前导的菌株中进行滴定。GlpP已得到纯化，凝胶漂移实验表明，它在体外可与glpD先导mRNA结合。GlpP与其他已知的抗终止子蛋白并不相似，但数据库搜索发现了一个与GlpP高度相似的大肠杆菌ORF。
The Bacillus subtilis glpD gene encodes glycerol-3-phosphate (G3P) dehydrogenase. Expression of glpD is mainly controlled by termination/antitermination of transcription at an inverted repeat in the glpD leader. Antitermination is mediated by the antiterminator protein GlpP in the presence of G3P. In this paper, interaction between GlpP and glpD leader mRNA in vivo and in vitro is reported. In vivo, the antiterminating effect of GlpP can be titrated in a strain carrying the glpD leader on a plasmid. GlpP has been purified and gel shift experiments have shown that it binds to glpD leader mRNA in vitro. GlpP is not similar to other known antiterminator proteins, but database searches have revealed an Escherichia coli ORF which has a high degree of similarity to GlpP.