Identification of corticosteroid-binding globulin in human milk: measurement with a filter disk assay.
Identification of corticosteroid-binding globulin in human milk: measurement with a filter disk assay.
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人乳中皮质类固醇结合球蛋白的鉴定：用滤盘测定进行测量。
DOI：
10.1210/jcem-42-6-1064
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
M. S. Khan
中科院分区：
文献类型：
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作者：
W. Rosner;P. C. Beers;T. Awan;M. S. Khan
我们已经研究了在分娩后的前100天内从女性获得的母乳样本中结合皮质醇的蛋白质的浓度和性质。建立了测定血浆皮质类固醇结合球蛋白（CBG）和乳汁皮质醇结合蛋白的滤纸片法。乳汁中CBG的浓度，以其结合皮质醇的能力表示，在分娩当天最高，约为0.80 mug/dl，在接下来的10天内下降至约为福尔斯。0.25 mug/dl，并在此后保持该水平。如果CBG的浓度相对于乳汁中血清白蛋白的浓度表示，则其从第1天到第3天增加，然后保持恒定。比较牛奶和血浆中的CBG，发现这两种蛋白质在葡聚糖凝胶、蔗糖梯度离心和聚丙烯酰胺凝胶电泳上共迁移。这两种蛋白质对cotrisol、孕酮、17-OH-孕酮和地塞米松具有相同的亲和力。此外，牛奶中CBG的结合活性被从血浆中分离的CBG产生的抗CBG抗体中和。与CBG不同，乳汁中皮质醇的浓度为0.8-3.5 μ g/dl，没有显示出作为获得样本的产后天数的函数的系统性变化。
We have studied the concentration and properties of a protein which binds cortisol in human milk in samples obtained from women during the first 100 days after delivery. A filter disk assay was developed both for the measurement of plasma corticosteroid-binding globulin (CBG) and for the cortisol-binding protein in milk. The concentration of CBG in milk, expressed as its capacity to bind cortisol, is highest on the day of delivery, ca, 0.80 mug/dl, falls over the next 10 days to ca. 0.25 mug/dl, and remains at that level thereafter. If the concentration of CBG is expressed relative to the concentration of serum albumin in milk, it increases from day 1 to day 3 and then remains constant. A detailed comparison of CBG derived from milk and plasma showed that the two proteins co-migrated on Sephadex, sucrose gradient ultracentrifugation, and polyacrylamide gel electrophoresis. The two proteins had the same affinity for cotrisol, progesterone, 17-OH-progesterone, and dexamethasone. Furthermore, the binding activity of CBG in milk was neutralized with anti-CBG antibodies raised against CBG isolated from plasma. Unlike CBG, the concentration of cortisol in milk, 0.8-3.5 mug/dl, showed no systematic variation as a function of the postpartum day on which the sample was obtained.