Evaluation of the diagnostic value of the immunoblotting and ELISA tests using recombinant Em18 antigen in human alveolar echinococcosis from Xingjiang China
Evaluation of the diagnostic value of the immunoblotting and ELISA tests using recombinant Em18 antigen in human alveolar echinococcosis from Xingjiang China
复制标题
重组Em18抗原免疫印迹和ELISA检测对新疆人肺泡包虫病的诊断价值评价
DOI：
10.3892/etm.2018.6555
复制
发表时间：
2018-10-01
影响因子：
2.7
通讯作者：
Lin, Ren-Yong
中科院分区：
文献类型：
--
作者：
Bi, Xiao-Juan;Shao, Yin-Mei;Lin, Ren-Yong
泡球蚴病（AE）是北方的一种流行病，特别是在中欧和中国西部。血清诊断对于AE患者很重要，尤其是在首次筛选期间。本研究纯化了重组Em 18-GST（rEm 18-GST），并采用免疫印迹（IB）和酶联免疫吸附试验（ELISA）检测了其对中国新疆泡球蚴病患者的诊断性能。收集了50例AE患者、222例囊型包虫病（CE）患者、158例其他无关感染患者和106例健康个体的血清样本。IB结果显示，47例AE和12例CE患者的血清样本为rEm 18阳性。然而，只有一个来自癌症患者的样本在IB中显示与rEm 18的交叉反应。总的敏感性为94%，总的特异性为96.58%。ELISA法检测rEm 18结果，46例AE患者血清为阳性，总敏感性为92%。总之，与包括计算机断层扫描、磁共振成像和正电子发射断层扫描在内的成像工具相比，rEm 18在AE血清学诊断中具有相当大的优势。
Alveolar echinococcosis (AE) is a prevalent epidemic in the northern hemisphere, especially in central Europe and western China. Serum diagnosis is important for patients with AE, especially during the first screening. The present study purified the recombinant Em18-GST (rEm18-GST), and detected its diagnostic performance in human alveolar echinococcosis patients of Xinjiang, China with immunoblotting (IB) and enzyme-linked immunosorbent assay (ELISA). Serum samples were collected from 50 patients with AE, 222 patients with cystic echinococcosis (CE), 158 patients with other unrelated infections and 106 healthy individuals. The IB results showed that serum samples of 47 patients with AE and 12 patients with CE were rEm18-positive. However, only one sample from patients with cancer showed a cross-reaction with rEm18 in IB. The overall sensitivity was 94%, and the total specificity was 96.58%. For the rEm18 results using ELISA, the sera of 46 patients with AE were positive, and the overall sensitivity was 92%. In conclusion, compared with imaging tools, including computed tomography, magnetic resonance imaging and positron emission tomography, rEm18 has considerable advantages for AE serodiagnosis.