Expression of uPAR mRNA in peripheral blood is a favourite marker for metastasis in gastric cancer cases
Expression of uPAR mRNA in peripheral blood is a favourite marker for metastasis in gastric cancer cases
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DOI：
10.1038/sj.bjc.6604806
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发表时间：
2009-01-13
影响因子：
8.8
通讯作者：
Mori, M.
中科院分区：
文献类型：
--
作者：
Kita, Y.;Fukagawa, T.;Mori, M.
尿激酶型纤溶酶原激活物受体（uPAR）在纤溶酶原激活级联反应中起核心作用，并参与细胞外基质降解、细胞迁移和侵袭。我们评估了胃癌患者骨髓（BM）和外周血（PB）中uPAR mRNA的表达水平和分离的肿瘤细胞（ITCs）的存在，并阐明其临床意义。我们通过实时定量逆转录聚合酶链反应（RT-PCR）评估了846例胃癌患者BM和PB中特异性uPAR mRNA的表达，以及三种上皮细胞标志物，癌胚抗原（CEA），细胞角蛋白（CK）-19和CK-7。骨髓和外周血中uPAR mRNA表达均显著高于正常对照组（P
Urokinase-type plasminogen activator receptor (uPAR) plays a central role in the plasminogen activation cascade and participates in extracellular matrix degradation, cell migration and invasion. We evaluated the expression level of uPAR mRNA and the presence of isolated tumour cells (ITCs) in bone marrow (BM) and peripheral blood (PB) in gastric cancer patients and clarified its clinical significance. We assessed specific uPAR mRNA expression by quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) in BM and PB in 846 gastric cancer patients as well as three epithelial cell markers, carcinoembryonic antigen (CEA), cytokeratin (CK)-19 and CK-7. The uPAR mRNA expression in bone marrow and peripheral blood expressed significantly higher than normal controls (P