Regulation of chicken embryonic growth hormone secretion by corticosterone and triiodothyronine
Regulation of chicken embryonic growth hormone secretion by corticosterone and triiodothyronine
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皮质酮和三碘甲状腺原氨酸对鸡胚胎生长激素分泌的调节
作者：
T. Porter;K. Dean
本文报道鸡胚发育第16天开始分化生长激素分泌细胞，这些生长激素细胞对生长激素释放激素和促甲状腺激素释放激素有反应。本实验评价了皮质酮和三碘甲状腺原氨酸（T3）对胚胎生长激素分泌的影响。在存在或不存在皮质酮或T3的情况下，对胚胎第（e）16、e18和e20天的垂体前叶细胞进行GH反向溶血空斑试验（RHPA）。皮质酮增加GH分泌从胚胎生长激素，E16和E18的效果特别明显。T3在e16时降低GH分泌，而在e18或e20时无显著影响。接下来，将垂体细胞与T3和皮质酮单独或组合进行RHPA。这些激素联合治疗抑制生长激素分泌E16，E18，和E20生长激素细胞低于基础条件下发现的水平。我们的结论是，皮质酮可以刺激生长激素分泌在体外测试的所有胚胎年龄。此外，T3可以抑制基础GH分泌的e16，和T3和皮质酮的组合可以抑制GH分泌在所有年龄。这些结果表明，生长激素分泌在鸡胚胎发育结束时可能是由内源性糖皮质激素和甲状腺激素的相互作用，孵化前增加。
We reported that growth hormone (GH)-secreting cells differentiated by d 16 of chick embryonic development and that these somatotrophs were responsive to GH-releasing hormone and thyrotropin-releasing hormone. The present, experiments evaluated effects of corticosterone and triiodothyronine (T3) on embryonic GH secretion. Anterior pituitary cells from embryonic day (e) 16, e18, and e20 were subjected to reverse hemolytic plaque assays (RHPAs) for GH in the absence or presence of corticosterone or T3. Corticosterone increased GH secretion from embryonic somatotrophs, an effect particularly evident on e16 and e18. T3 decreased GH secretion on e16, while no effect of T3 was significant on e18 or e20. Next, pituitary cells were subjected to RHPAs with T3 and corticosterone alone or in combination. Combined treatment with these hormones suppressed GH secretion from e16, e18, and e20 somatotrophs to levels below those found under basal conditions. We conclude that corticosterone can stimulate GH secretion in vitro at all embryonic ages tested. Furthermore, T3 can suppress basal GH secretion on e16, and the combination of T3 and corticosterone can suppress GH secretion at all ages. These findings indicate that GH secretion during the end of chicken embryonic development may be regulated by the interactions of endogenous glucocorticoids and thyroid hormones that increase prior to hatching.
合成人胰腺（肿瘤）GH 释放因子和生长抑素、三碘甲腺原氨酸和地塞米松对体外 GH 分泌的影响。
影响因子：
4.8
作者：
Vale,W;Vaughan,J;Yamamoto,G;Spiess,J;Rivier,J
通讯作者：
Rivier,J
儿茶酚胺、前列腺素和甲状腺激素对体外鸡垂体细胞分泌生长激素的影响。
影响因子：
2.1
作者：
Donoghue,DJ;Perez,FM;Diamante,BS;Malamed,S;Scanes,CG
通讯作者：
Scanes,CG