Genetic analysis of P-TEFb function via heterologous nucleic acid tethering systems
Genetic analysis of P-TEFb function via heterologous nucleic acid tethering systems
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DOI：
10.1016/j.ymeth.2009.04.006
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发表时间：
2009-08-01
期刊：
影响因子：
4.8
通讯作者：
Peterlin, B. Matija
中科院分区：
文献类型：
--
作者：
Li, Qintong;Peterlin, B. Matija
最近的全球遗传分析表明，转录延伸水平上的基因表达调控是真核生物的一个共同特征。正转录延伸因子 P-TEFb 在此过程中发挥着关键作用。 P-TEFb 是一种细胞周期蛋白依赖性激酶，它控制可进入生产性延伸的 RNA 聚合酶 II (RNAP II) 的部分。虽然 P-TEFb 的生化特性及其相关因子已在体外得到广泛表征，但其体内功能仍不清楚。在本文中，我们描述了可用于检查通过 P-TEFb 发挥作用的转录因子的各种异源核酸束缚系统。 (C) 2009 Elsevier Inc. 保留所有权利。
Recent global genetic analyses demonstrated that the regulation of gene expression at the level of transcription elongation is a common feature in eukaryotes. The positive transcription elongation factor P-TEFb plays a critical role in this process. P-TEFb is a cyclin-dependent kinase, which controls the fraction of RNA polymerase II (RNAP II) that can enter productive elongation. While the biochemical properties of P-TEFb and its associated factors have been characterized extensively in vitro, its function in vivo remains less well understood. In this paper, we describe various heterologous nucleic acid tethering systems that can be used to examine transcription factors that function via P-TEFb. (C) 2009 Elsevier Inc. All rights reserved.