CHARACTERIZATION OF A NEW VIRUS-NEUTRALIZING EPITOPE THAT DENOTES A SEQUENTIAL DETERMINANT ON THE RABIES VIRUS GLYCOPROTEIN
CHARACTERIZATION OF A NEW VIRUS-NEUTRALIZING EPITOPE THAT DENOTES A SEQUENTIAL DETERMINANT ON THE RABIES VIRUS GLYCOPROTEIN
复制标题
DOI：
10.1099/0022-1317-70-2-291
复制
发表时间：
1989-02-01
影响因子：
3.8
通讯作者：
OSTERHAUS, ADME
中科院分区：
文献类型：
--
作者：
BUNSCHOTEN, H;GORE, M;OSTERHAUS, ADME
两个新的单克隆抗体（MAbs）来自小鼠免疫的Pitman-Moore（PM）株狂犬病病毒被用来识别和表征狂犬病病毒糖蛋白上的两个独特的抗原决定簇。在竞争性结合研究和中和抗性变异病毒的比较抗原分析中，新产生的单克隆抗体6- 15 C4将其中一个决定簇（定义了狂犬病毒糖蛋白上的额外抗原位点）描绘为一个独特的表位。将这两个抗原决定簇与之前描述的五个抗原位点进行比较，这些抗原位点结合攻毒病毒标准品（CVS）和Evelyn-Rokitnicki-Abelseth（ERA）毒株糖蛋白上的病毒中和抗体。本通讯中提供的结果表明，6- 15 C4表位是狂犬病病毒糖蛋白中描述的第一个表位，其不依赖于糖蛋白的天然构象来结合病毒中和抗体。这些数据表明，有可能产生针对狂犬病的合成肽疫苗。
Two new monoclonal antibodies (MAbs) derived from mice immunized with the Pitman-Moore (PM) strain of rabies virus were used to identify and characterize two unique antigenic determinants on the rabies virus glycoprotein. One of the determinants, which defined an additional antigenic site on the rabies virus glycoprotein, was delineated as a distinct epitope by the newly generated MAb, 6-15C4, in competitive binding studies and by comparative antigenic analysis of neutralizatin-resistant variant viruses. Both antigenic determinants were compared with the five previously described antigenic sites which bind virus-neutralizing antibodies on the challenge virus standard (CVS) and Evelyn-Rokitnicki-Abelseth (ERA) strain glycoproteins. The results presented in this communication show that the 6-15C4 epitope is the first epitope described in the rabies virus glycoprotein that does not depend on the native conformation of the glycoprotein for binding virus-neutralizing antibody. These data suggest that it may be possible to generate a synthetic peptide vaccine against rabies.