Inhibition and enhancement of odorant-induced cAMP accumulation in rat olfactory cilia by antibodies directed against G alpha S/olf- and G alpha i-protein subunits.
Inhibition and enhancement of odorant-induced cAMP accumulation in rat olfactory cilia by antibodies directed against G alpha S/olf- and G alpha i-protein subunits.
复制标题
通过针对 G α S/olf 和 G α i 蛋白亚基的抗体抑制和增强大鼠嗅纤毛中气味剂诱导的 cAMP 积累。
DOI：
10.1016/s0014-5793(98)00368-8
复制
发表时间：
1998
期刊：
影响因子：
3.5
通讯作者：
Vodyanoy,V
中科院分区：
文献类型：
--
作者：
Sinnarajah,S;Ezeh,PI;Pathirana,S;Moss,AG;Morrison,EE;Vodyanoy,V
针对Gαs/olf的抗体可以抑制气味诱导的cAMP积累。相反，针对Gαi-亚基的抗体引起气味诱导的cAMP积累的强烈增强。Western印迹和免疫电镜显示大鼠纤毛制备物中存在GαS/olf-和Gαi-亚基。嗅纤毛中腺苷酸环化酶活性的刺激性和抑制性气味诱导调节的存在可能表明，不同气味刺激的初始整合开始于同一效应酶的初级反应水平。
The odorant‐induced accumulation of cAMP can be inhibited by antibodies directed against Gαs/olf. In contrast, antibodies raised against Gαi‐subunits caused a strong enhancement of the odorant‐induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence of both GαS/olf‐ and Gαi‐subunits in rat cilia preparations. The existence of both stimulatory and inhibitory odorant‐induced regulation of adenylyl cyclase activity in olfactory cilia may indicate that an initial integration of different odorant stimuli begins at the level of primary reactions in the same effector enzyme.