Changes in peptidergic neurotransmission during postoperative ileus in rat circular jejunal muscle
Changes in peptidergic neurotransmission during postoperative ileus in rat circular jejunal muscle
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DOI：
10.1111/nmo.12275
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发表时间：
2014-03-01
影响因子：
3.5
通讯作者：
Kasparek，M. S.
中科院分区：
文献类型：
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作者：
Goetz，B.;Benhaqi，P.;Kasparek，M. S.
背景我们的目的是探索术后肠梗阻 (POI) 期间血管活性肠肽 (VIP) 和 P 物质 (Sub P) 神经传递的未知变化。方法分五组研究大鼠环形空肠肌条的收缩活性（n = 6/组）：初始对照组、剖腹手术后 12 小时和 3 天的假手术对照组，以及诱导后 12 小时和 3 天的大鼠。兴趣点。使用不同的拮抗剂研究了 VIP (10−10–10−7M)、Sub P (3 × 10−10–3 × 10−7M) 和电场刺激（EFS，研究神经递质的内源性释放）的剂量反应。研究了肠壁中肠道转运、炎症细胞和 VIP 和 Sub P 的免疫反应性，并测定了脑干迷走神经传入和传出核中细胞芬克尔骨肉瘤的表达。关键结果以肠道转运延迟和壁内炎症为特征的术后肠梗阻与 VIP 对收缩活动的抑制作用增强有关。观察到 Sub P 的双相影响，其对收缩性的兴奋性潜力在 12 小时时下降，随后在术后 3 天增加。肠梗阻动物对 EFS 的抑制反应增加，而兴奋反应减少。所有术后动物的 VIP 表达均增加，而只有肠梗阻诱导后 3 天的动物肌间神经丛中的 Sub P 表达增加。这些变化与脑干中迷走神经传入核的激活相关，但与迷走传出核的激活无关。 结论与推论 POI 期间，VIP 和 Sub P 的肽能神经传递发生特定的时间依赖性变化，与迷走神经传入激活相关，但与迷走神经传出通路的激活无关。
BackgroundOur aim was to explore unknown changes in neurotransmission with vasoactive intestinal peptide (VIP) and Substance P (Sub P) during postoperative ileus (POI).MethodsContractile activity of rat circular jejunal muscle strips was studied in five groups (n = 6/group): Naïve controls, sham controls 12 h and 3 days after laparotomy, and rats 12 h, 3 days after induction of POI. Dose‐responses to VIP (10−10–10−7M), Sub P (3 × 10−10–3 × 10−7M), and electrical field stimulation (EFS, to study endogenous release of neurotransmitters) were studied with different antagonists. Intestinal transit, inflammatory cells and immunoreactivity for VIP and Sub P were investigated in the bowel wall and cellular Finkel osteo sarcoma expression was determined in vagal afferent and efferent nuclei of the brainstem.Key ResultsPostoperative ileus characterized by delayed intestinal transit and intramural inflammation was associated with an increased inhibitory effect of VIP on contractile activity. A biphasic impact was observed for Sub P with a decrease in its excitatory potential on contractility at 12 h, followed by a later increase 3 days postoperatively. Inhibitory response to EFS was increased, whereas the excitatory response decreased in ileus animals. VIP expression was increased in all postoperative animals while only animals 3 days after ileus induction showed increased Sub P expression in the myenteric plexus. These changes were associated with an activation of afferent but not efferent vagal nuclei in the brain stem.Conclusions & InferencesSpecific, time‐dependent changes in peptidergic neurotransmission with VIP and Sub P occur during POI that are associated with vagal afferent activation, but are independent of the activation of efferent vagal pathways.