ZmMTOPVIB Enables DNA Double-Strand Break Formation and Bipolar Spindle Assembly during Maize Meiosis
ZmMTOPVIB Enables DNA Double-Strand Break Formation and Bipolar Spindle Assembly during Maize Meiosis
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ZmMTOPVIB 在玉米减数分裂过程中实现 DNA 双链断裂形成和双极纺锤体组装。
DOI：
10.1104/pp.20.00933
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发表时间：
2020-12-01
期刊：
影响因子：
7.4
通讯作者：
He, Yan
中科院分区：
文献类型：
--
作者：
Jing, Ju-Li;Zhang, Ting;He, Yan
编码拓扑异构酶MTOPVIB在减数分裂双链断裂形成和双极纺锤体组装中的双重作用，在单子叶植物中是进化保守的。减数分裂TopoVI B亚基（MTopVIB）在小鼠（Mus musus）、拟南芥（Arabidopsis thaliana）和水稻（Oryza sativa）的双链断裂形成中起着至关重要的作用，最近的研究表明，水稻MTopVIB在减数分裂双纺锤体组装中也起着意想不到的作用，突出了MTopVIB在水稻减数分裂中的多重功能。在这项工作中，我们鉴定了玉米减数分裂TopVIB （Zea mays; ZmMTOPVIB）。ZmmtopVIB突变体植株营养生长正常，但雌雄不育。减数分裂双链断裂在突变减数细胞中消失。尽管轴向元件正常组装，突变体表现出严重影响突触和破坏同源配对。重要的是，我们发现在ZmmtopVIB中双极性主轴装配也受到影响，导致三合一和多合一的形成。总之，我们的研究结果表明，ZmMTOPVIB在双链断裂形成和同源重组中起着关键作用。此外，我们的研究结果表明，MTOPVIB在双极主轴装配中的功能可能在不同的单子体中是保守的。
The dual roles of the topoisomerase-encoding MTOPVIB in meiotic double-strand break formation and bipolar spindle assembly and are evolutionarily conserved in monocot plants. The meiotic TopoVI B subunit (MTopVIB) plays an essential role in double-strand break formation in mouse (Mus musculus), Arabidopsis (Arabidopsis thaliana), and rice (Oryza sativa), and recent work reveals that rice MTopVIB also plays an unexpected role in meiotic bipolar spindle assembly, highlighting multiple functions of MTopVIB during rice meiosis. In this work, we characterized the meiotic TopVIB in maize (Zea mays; ZmMTOPVIB). The ZmmtopVIB mutant plants exhibited normal vegetative growth but male and female sterility. Meiotic double-strand break formation was abolished in mutant meiocytes. Despite normal assembly of axial elements, mutants showed severely affected synapsis and disrupted homologous pairing. Importantly, we showed that bipolar spindle assembly was also affected in ZmmtopVIB, resulting in triad and polyad formation. Overall, our results demonstrate that ZmMTOPVIB plays critical roles in double-strand break formation and homologous recombination. In addition, our results suggest that the function of MTOPVIB in bipolar spindle assembly is likely conserved across different monocots.