Crystal-Structure-Guided Design of Self-Assembling RNA Nanotriangles.
Crystal-Structure-Guided Design of Self-Assembling RNA Nanotriangles.
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DOI：
10.1002/anie.201600233
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发表时间：
2016-03-14
期刊：
影响因子：
--
通讯作者：
Hermann T
中科院分区：
文献类型：
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作者：
Boerneke MA;Dibrov SM;Hermann T
RNA纳米技术利用RNA结构基序来构建通过选择性碱基对相互作用组装的纳米尺寸架构。在这里，我们报告了晶体结构指导的设计高度稳定的RNA纳米三角形，自组装合作短寡核苷酸。在2.6 nm处测定的81个核苷酸纳米三角形的晶体结构揭示了完全由双链RNA制成的最小的圆形封闭纳米物体。纳米三角形结构的组装涉及源自配体响应性RNA开关的RNA角基序，其提供了控制自组装和解离的机会。RNA纳米结构设计和表征：两个短的寡核苷酸在溶液中协同自组装，形成完全由双链RNA组成的最小的圆形闭合纳米三角形。这种纳米物体形成晶体，其结构通过X射线衍射以2.6 nm的分辨率确定。
RNA nanotechnology harnesses RNA structural motifs to build nano-sized architectures which assemble through selective base pair interactions. Here, we report the crystal structure-guided design of highly stable RNA nanotriangles that self-assemble cooperatively from short oligonucleotides. The crystal structure of an 81 nucleotide nanotriangle determined at 2.6 Å reveals the yet smallest circularly closed nano-object made entirely of double-stranded RNA. Assembly of the nanotriangle architecture involved RNA corner motifs that were derived from ligand-responsive RNA switches which offer the opportunity to control self-assembly and dissociation. RNA nanostructure design and characterization: Two short oligonucleotides self-assemble cooperatively in solution to form the yet smallest circularly closed nanotriangle made entirely of double-stranded RNA. This nano-object formed crystals, and its structure was determined by X-ray diffraction at 2.6 Å resolution.