Hsp90 is involved in the formation of P-bodies and stress granules
Hsp90 is involved in the formation of P-bodies and stress granules
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DOI：
10.1016/j.bbrc.2011.03.088
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发表时间：
2011-04-22
影响因子：
3.1
通讯作者：
Minami, Yasufumi
中科院分区：
文献类型：
--
作者：
Matsumoto, Ken;Minami, Michiko;Minami, Yasufumi
在此之前，我们发现用Hsp 90抑制剂格尔德霉素（GA）处理细胞导致加工体（P-体）数量的大幅减少，并且还改变了应激颗粒的大小和亚细胞定位。这些结果表明，伴侣活性的热休克蛋白90参与的P-体和应激颗粒的形成。为了验证这些观察结果，我们研究了另一种热休克蛋白90抑制剂根赤霉素（RA）是否影响P-体和应激颗粒。RA治疗降低了Hsp 90客户蛋白Argonaute 2的水平和P体的数量。热休克后，RA处理的细胞中虽然仍有应激颗粒聚集，但它们比未处理的细胞中更小，更分散于细胞质中。此外，eIF 4 E和eIF 4 E-转运蛋白被选择性地从RA处理的细胞中的应激颗粒中解离。这些观察结果与我们以前的工作中用GA治疗后获得的结果相当。因此，我们得出结论，废除伴侣活性的热休克蛋白90影响P-体的形成和完整的应力颗粒。(C)2011 Elsevier Inc. All rights reserved.
Previously, we found that treatment of cells with the Hsp90 inhibitor geldanamycin (GA) leads to a substantial reduction in the number of processing bodies (P-bodies), and also alters the size and subcellular localization of stress granules. These findings imply that the chaperone activity of Hsp90 is involved in the formation of P-bodies and stress granules. To verify these observations, we examined whether another Hsp90 inhibitor radicicol (RA) affected P-bodies and stress granules. Treatment with RA reduced the level of the Hsp90 client protein Argonaute 2 and the number of P-bodies. Although stress granules still assembled in RA-treated cells upon heat shock, they were smaller and more dispersed in the cytoplasm than those in untreated cells. Furthermore eIF4E and eIF4E-transporter were dissociated selectively from stress granules in RA-treated cells. These observations were comparable to those obtained upon treatment with GA in our previous work. Thus, we conclude that abrogation of the chaperone activity of Hsp90 affects P-body formation and the integrity of stress granules. (C) 2011 Elsevier Inc. All rights reserved.