FOXP3+T regulatory cells in lesions of oral lichen planus correlated with disease activity
FOXP3+T regulatory cells in lesions of oral lichen planus correlated with disease activity
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DOI：
10.1111/j.1601-0825.2009.01608.x
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发表时间：
2010-01-01
期刊：
影响因子：
3.8
通讯作者：
Cheng, B.
中科院分区：
文献类型：
--
作者：
Tao, X-a;Xia, J.;Cheng, B.
目的：探讨FOXP 3 + T细胞在口腔扁平苔藓（oral lichen planus，OLP）患者皮损中的表达与疾病活动性的关系。结果：正常对照组中仅检测到少量FOXP 3+细胞，而病变组中FOXP 3+细胞明显增多（n = 20，40.99 ± 24.68个细胞/高倍视野- hpf）。此外，网状OLP中FOXP 3 + Treg的频率（n = 7，63.6 +/- 23.2个细胞/hpf）显著高于糜烂性OLP（n = 13，28.8 +/- 16.8个细胞/hpf，P = 0.001）。此外，FOXP 3 + Treg数量与疾病活动度呈负相关（相关系数=-0.557，P = 0.013）。治疗后患者外周血中FOXP 3 + Treg的比例显著升高（1.39 ± 0.71%vs4.91 ± 1.59%）。
Objective:The aim of this study was to determine the correlation between the number of FOXP3+ T cell in lesions and the disease activity of patients with oral lichen planus (OLP).Materials and Methods:The expression of FOXP3 was investigated using immunohistochemical staining and real-time RT-PCR in 23 OLP lesions and 12 controls. Changes of FOXP3+ Treg in peripheral blood from three patients' pre and post-treatment were assessed using flow cytometry.Results:Few FOXP3+ cells were detected in controls, but an increased number of FOXP3+ cells were observed in lesions (n = 20, 40.99 +/- 24.68 cells per high-power field - hpf). Furthermore, the frequency of FOXP3+ Treg in reticular OLP (n = 7, 63.6 +/- 23.2 cells per hpf) was significantly higher than that in erythematous/erosive OLP (n = 13, 28.8 +/- 16.8 cells per hpf, P = 0.001). In addition, negative correlation was found between the number of FOXP3+ Treg and disease activity (correlation oefficient = -0.557, P = 0.013). The proportion of FOXP3+ Treg showed remarkable increase in peripheral blood from patients after treatment (1.39 +/- 0.71%vs 4.91 +/- 1.59%).Conclusions:These data indicated that FOXP3+ Treg were involved in the pathogenesis of OLP and correlated with disease's subtype and activity.