Intrinsic anti-inflammatory properties in the serum of two species of deep-diving seal
Intrinsic anti-inflammatory properties in the serum of two species of deep-diving seal
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DOI：
10.1242/jeb.178491
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发表时间：
2018-07-01
影响因子：
2.8
通讯作者：
Hindle, Allyson G.
中科院分区：
文献类型：
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作者：
Bagchi, Aranya;Batten, Annabelle J.;Hindle, Allyson G.
威德尔海豹和象海豹是深潜哺乳动物，它们依靠肺塌陷来限制氮的吸收，防止减压损伤。反复塌陷和再扩张使肺部暴露于多种应激源，包括缺血-再灌注、肺泡剪切应力和炎症。然而，没有证据表明潜水会损害这些物种的肺功能。为了研究深潜海豹的潜在保护策略，我们检测了暴露于脂多糖（LPS）的海豹全血的炎症反应。一种强效的内毒素。与健康的人类血液相比，暴露在LPS下健康的北象海豹和威德尔海豹血液中产生的白细胞介素-6 （IL6）细胞因子降低了50 ~ 500倍。与LPS暴露的威德尔海豹全血中IL6蛋白的产量增加了6倍相比，在标准细胞培养条件下，使用补充胎牛血清（FBS）的培养基，分离的威德尔海豹外周血单个核细胞产生了强大的LPS反应（类似于300倍）。诱导116 mRNA的表达以及IL6、IL8、IL10的产生。用等量的自体海豹血清替代FBS可降低KC-like和TNFa。威德尔海豹血清还能减弱LPS对RAW 267.4小鼠巨噬细胞的炎症反应。皮质醇水平和添加血脂对培养细胞的细胞因子反应没有影响。这些数据表明海豹血清具有抗炎特性，可以保护深潜者免受自然发生的炎症挑战，如潜水引起的缺氧再氧合和肺衰竭。
Weddell and elephant seals are deep-diving mammals, which rely on lung collapse to limit nitrogen absorption and prevent decompression injury. Repeated collapse and re-expansion exposes the lungs to multiple stressors, including ischemia-reperfusion, alveolar shear stress and inflammation. There is no evidence, however, that diving damages pulmonary function in these species. To investigate potential protective strategies in deep-diving seals, we examined the inflammatory response of seal whole blood exposed to lipopolysaccharide (LPS). a potent endotoxin. Interleukin-6 (IL6) cytokine production elicited by LPS exposure was 50 to 500 times lower in blood of healthy northern elephant seals and Weddell seals compared with that of healthy human blood. In contrast to the similar to 6x increased production of IL6 protein from LPS-exposed Weddell seal whole blood, isolated Weddell seal peripheral blood mononuclear cells, under standard cell culture conditions using medium supplemented with fetal bovine serum (FBS), produced a robust LPS response (similar to 300x). Induction of 116 mRNA expression as well as production of IL6, IL8, IL10. KC-like and TNFa were reduced by substituting FBS with an equivalent amount of autologous seal serum. Weddell seal serum also attenuated the inflammatory response of RAW 267.4 mouse macrophage cells exposed to LPS. Cortisol level and the addition of serum lipids did not impact the cytokine response in cultured cells. These data suggest that seal serum possesses anti-inflammatory properties, which may protect deep divers from naturally occurring inflammatory challenges such as dive-induced hypoxia-reoxygenation and lung collapse.