Microfracture and bone morphogenetic protein 7 (BMP-7) synergistically stimulate articular cartilage repair
Microfracture and bone morphogenetic protein 7 (BMP-7) synergistically stimulate articular cartilage repair
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DOI：
10.1016/j.joca.2006.04.004
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发表时间：
2006-11-01
影响因子：
7
通讯作者：
Chiu, M.
中科院分区：
文献类型：
--
作者：
Kuo, A. C.;Rodrigo, J. J.;Chiu, M.
目的：微骨折用于治疗关节软骨损伤，但导致纤维软骨而不是天然透明关节软骨的形成。由于骨形态发生蛋白7（BMP-7）诱导软骨分化，我们假设形态发生蛋白的加入将改善微骨折产生的修复组织。我们确定了这两种治疗方法单独和组合的质量和数量的修复组织形成的模型中的全层关节软骨损伤的青少年rabbits.Design：全层缺损的髌骨沟的关节软骨的40，15周龄的兔子。然后将八只动物分配到（1）不进一步治疗（对照）、（2）微骨折、（3）BMP-7、（4）胶原海绵中的BMP-7微骨折（组合治疗）和（5）胶原海绵微骨折。24周后，在39周龄时处死动物。愈合的程度通过确定修复组织的厚度和表面积来定量。修复组织的质量通过使用国际软骨修复学会视觉组织学评估Scale.Results：与对照组相比，BMP-7单独增加修复组织的量，而不影响修复组织的质量。微骨折术可提高修复组织的数量和表面光滑度。与单一治疗相比，微骨折和BMP-7的组合增加了修复tissue.Conclusions的质量和数量：微骨折和BMP-7协同作用，以刺激软骨修复，导致更大量的修复组织，更接近本地透明关节软骨。(C)2006年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: Microfracture is used to treat articular cartilage injuries, but leads to the formation of fibrocartilage rather than native hyaline articular cartilage. Since bone morphogenetic protein 7 (BMP-7) induces cartilage differentiation, we hypothesized that the addition of the morphogen would improve the repair tissue generated by microfracture. We determined the effects of these two treatments alone and in combination on the quality and quantity of repair tissue formed in a model of full-thickness articular cartilage injury in adolescent rabbits.Design: Full-thickness defects were made in the articular cartilage of the patellar grooves of forty, 15-week-old rabbits. Eight animals were then assigned to (1) no further treatment (control), (2) microfracture, (3) BMP-7, (4) microfracture with BMP-7 in a collagen sponge (combination treatment), and (5) microfracture with a collagen sponge. Animals were sacrificed after 24 weeks at 39 weeks of age. The extent of healing was quantitated by determining the thickness and the surface area of the repair tissue. The quality of the repair tissue was determined by grading specimens using the International Cartilage Repair Society Visual Histological Assessment Scale.Results: Compared to controls, BMP-7 alone increased the amount of repair tissue without affecting the quality of repair tissue. Microfracture improved both the quantity and surface smoothness of repair tissue. Compared to either single treatment, the combination of microfracture and BMP-7 increased both the quality and quantity of repair tissue.Conclusions: Microfracture and BMP-7 act synergistically to stimulate cartilage repair, leading to larger amounts of repair tissue that more closely resembles native hyaline articular cartilage. (C) 2006 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.