Distinct forms of the beta subunit of GTP-binding regulatory proteins identified by molecular cloning.
Distinct forms of the beta subunit of GTP-binding regulatory proteins identified by molecular cloning.
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通过分子克隆鉴定出 GTP 结合调节蛋白 β 亚基的不同形式。
DOI：
10.1073/pnas.84.11.3792
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发表时间：
1987
影响因子：
11.1
通讯作者：
Simon,MI
中科院分区：
文献类型：
--
作者：
Fong,HK;Amatruda3rd,TT;Birren,BW;Simon,MI
鸟嘌呤核苷酸结合调节蛋白的两个不同的β亚基已通过cDNA克隆鉴定出来，被称为β1和β2亚基。牛转导蛋白β亚基（β1）先前已被克隆。我们现在已经从牛肾上腺、牛脑和人骨髓性白血病细胞系 HL-60 中分离和分析了编码 β2 亚基的 cDNA 克隆。含有 340 个残基的 Mr 37,329 β 2 蛋白在预测的氨基酸序列中与 β 1 具有 90% 的同一性，并且它也被组织为一系列重复的同源片段。编码牛 β2 亚基的主要 mRNA 长度为 1.7 KB。在所有检查的组织中，其表达水平均低于 β1 亚基 mRNA。 beta 1 和 beta 2 信息在克隆的人类细胞系中表达。 cDNA 探针与牛 DNA 的杂交表明β1 和β2 由不同的基因编码。牛和人β2亚基的氨基酸序列是相同的，牛和人β1亚基的氨基酸序列也是相同的。这种进化保守性表明两个β亚基在信号转导过程中具有不同的作用。
Two distinct beta subunits of guanine nucleotide-binding regulatory proteins have been identified by cDNA cloning and are referred to as beta 1 and beta 2 subunits. The bovine transducin beta subunit (beta 1) has been cloned previously. We have now isolated and analyzed cDNA clones that encode the beta 2 subunit from bovine adrenal, bovine brain, and a human myeloid leukemia cell line, HL-60. The 340-residue Mr 37,329 beta 2 protein is 90% identical with beta 1 in predicted amino acid sequence, and it is also organized as a series of repetitive homologous segments. The major mRNA that encodes the bovine beta 2 subunit is 1.7 kilobases in length. It is expressed at lower levels than beta 1 subunit mRNA in all tissues examined. The beta 1 and beta 2 messages are expressed in cloned human cell lines. Hybridization of cDNA probes to bovine DNA showed that beta 1 and beta 2 are encoded by separate genes. The amino acid sequences for the bovine and human beta 2 subunit are identical, as are the amino acid sequences for the bovine and human beta 1 subunit. This evolutionary conservation suggests that the two beta subunits have different roles in the signal transduction process.