Bluetongue virus serotype 8-associated congenital hydranencephaly in calves
Bluetongue virus serotype 8-associated congenital hydranencephaly in calves
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DOI：
10.1111/j.1865-1682.2008.01034.x
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发表时间：
2008-09-01
影响因子：
4.3
通讯作者：
De Clercq, K.
中科院分区：
文献类型：
--
作者：
Vercauteren, G.;Miry, C.;De Clercq, K.
无脑积水，几乎完全没有脑实质，由感染改良的活蓝舌病毒（BTV）穿过胎盘引起的，以前曾在美国和南非的绵羊和牛中报道过。目前的研究描述了29例积水性无脑畸形的牛胎儿和“假”小牛长达3个月的年龄在比利时与自然BTV血清型8感染非常早期的妊娠。其余脑实质的组织学检查显示神经组织中度至重度萎缩。观察到的病变支持BTV诱导的前体细胞破坏的假设。然而，在几头小牛中也观察到T淋巴细胞轻微浸润小静脉和小动脉壁（血管炎），这似乎是至少部分病变的原因。采用BTV特异性实时RT-PCR方法在15只动物中检测到蓝舌病病毒RNA，与血液和脾脏样品相比，脑组织中的成功率要高得多。鸡胚中的病毒分离不成功。总之，犊牛积水性无脑畸形可能与自然野生型BTV-8宫内感染有关。
Hydranencephaly, the almost complete absence of the cerebral parenchyma, induced by infection with modified live bluetongue virus (BTV) crossing the placenta has previously been reported in sheep and rarely in cattle in the USA and in South Africa. The current study describes 29 cases of hydranencephaly in bovine foetuses and 'dummy' calves up to 3 months of age in Belgium associated with natural BTV serotype 8 infection very early in gestation. Histological examination of the remaining cerebral parenchyma showed moderate to severe atrophy of the neural tissue. The lesions observed support the hypothesis of BTV-induced destruction of precursor cells. However, in several calves a slight infiltration of the walls of venules and arterioles with T lymphocytes (vasculitis) was observed as well, which seems to be responsible for at least some of the lesions. Bluetongue viral RNA was detected in 15 animals using a BTV-specific real-time RT-PCR with a much higher success rate in brain tissues compared with blood and spleen samples. Virus isolation in embryonated eggs was unsuccessful. In conclusion, hydranencephaly in calves can be associated with natural wild-type BTV-8 intra-uterine infection.